A highly differentiated region of wheat chromosome 7AL encodes a Pm1a immune receptor that recognizes its corresponding AvrPm1a effector from Blumeria graminis

0545701 - ÚEB 2022 RIV GB eng J - Článek v odborném periodiku
Hewitt, T. - Müller, M. C. - Molnár, István - Mascher, M. - Holušová, Kateřina - Šimková, Hana - Kunz, L. - Zhang, J. - Li, J. - Bhatt, D. - Sharma, R. - Schudel, S. - Yu, G. - Steuernagel, B. - Periyannan, S. - Wulff, B. - Ayliffe, M.A. - McIntosh, R. - Keller, B. - Lagudah, E. - Zhang, P.
A highly differentiated region of wheat chromosome 7AL encodes a Pm1a immune receptor that recognizes its corresponding AvrPm1a effector from Blumeria graminis.
New Phytologist. Roč. 229, č. 5 (2021), s. 2812-2826. ISSN 0028-646X. E-ISSN 1469-8137
Grant CEP: GA MŠMT(CZ) EF16_019/0000827
Institucionální podpora: RVO:61389030
Klíčová slova: AvrPm effectors * Bgt * Blumeria graminis f. sp. tritici * chromosome sequencing * EMS mutagenesis * nlr * Triticum aestivum
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 10.323, rok: 2021
Způsob publikování: Open access
http://doi.org/10.1111/nph.17075

Pm1a, the first powdery mildew resistance gene described in wheat, is part of a complex resistance (R) gene cluster located in a distal region of chromosome 7AL that has suppressed genetic recombination. A nucleotide-binding, leucine-rich repeat (NLR) immune receptor gene was isolated using mutagenesis and R gene enrichment sequencing (MutRenSeq). Stable transformation confirmed Pm1a identity which induced a strong resistance phenotype in transgenic plants upon challenge with avirulent Blumeria graminis (wheat powdery mildew) pathogens. A high-density genetic map of a B. graminis family segregating for Pm1a avirulence combined with pathogen genome resequencing and RNA sequencing (RNAseq) identified AvrPm1a effector gene candidates. In planta expression identified an effector, with an N terminal Y/FxC motif, that induced a strong hypersensitive response when co-expressed with Pm1a in Nicotiana benthamiana. Single chromosome enrichment sequencing (ChromSeq) and assembly of chromosome 7A suggested that suppressed recombination around the Pm1a region was due to a rearrangement involving chromosomes 7A, 7B and 7D. The cloning of Pm1a and its identification in a highly rearranged region of chromosome 7A provides insight into the role of chromosomal rearrangements in the evolution of this complex resistance cluster.
Trvalý link: http://hdl.handle.net/11104/0322368