Počet záznamů: 1  

Influence of Drying Method and Argon Plasma Modification of Bacterial Nanocellulose on Keratinocyte Adhesion and Growth

  1. 1.
    0545468 - FGÚ 2022 RIV CH eng J - Článek v odborném periodiku
    Kutová, A. - Staňková, Ľubica - Vejvodová, K. - Kvítek, O. - Vokatá, B. - Fajstavr, D. - Kolská, Z. - Brož, Antonín - Bačáková, Lucie - Švorčík, V.
    Influence of Drying Method and Argon Plasma Modification of Bacterial Nanocellulose on Keratinocyte Adhesion and Growth.
    Nanomaterials. Roč. 11, č. 8 (2021), č. článku 1916. E-ISSN 2079-4991
    Grant CEP: GA ČR(CZ) GA20-01641S
    Institucionální podpora: RVO:67985823
    Klíčová slova: bacterial nanocellulose * lyophilization * plasma modification * cell adhesion
    Obor OECD: Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
    Impakt faktor: 5.719, rok: 2021
    Způsob publikování: Open access
    https://www.mdpi.com/2079-4991/11/8/1916

    Due to its nanostructure, bacterial nanocellulose (BC) has several advantages over plant cellulose, but it exhibits weak cell adhesion. To overcome this drawback, we studied the drying method of BC and subsequent argon plasma modification (PM). BC hydrogels were prepared using the Komagataeibacter sucrofermentans (ATCC 700178) bacteria strain. The hydrogels were transformed into solid samples via air-drying (BC-AD) or lyophilization (BC-L). The sample surfaces were then modified by argon plasma. SEM revealed that compared to BC-AD, the BC-L samples maintained their nanostructure and had higher porosity. After PM, the contact angle decreased while the porosity increased. XPS showed that the O/C ratio was higher after PM. The cell culture experiments revealed that the initial adhesion of human keratinocytes (HaCaT) was supported better on BC-L, while the subsequent growth of these cells and final cell population density were higher on BC-AD. The PM improved the final colonization of both BC-L and BC-AD with HaCaT, leading to formation of continuous cell layers. Our work indicates that the surface modification of BC renders this material highly promising for skin tissue engineering and wound healing.
    Trvalý link: http://hdl.handle.net/11104/0322156

     
     
Počet záznamů: 1  

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