DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification

0545150 - ÚŽFG 2022 RIV GB eng J - Článek v odborném periodiku
Bora, P. - Gahurová, Lenka - Hauserová, A. - Stiborová, M. - Collier, R. - Potěšil, D. - Zdráhal, Z. - Bruce, A. W.
DDX21 is a p38-MAPK-sensitive nucleolar protein necessary for mouse preimplantation embryo development and cell-fate specification.
Open Biology. Roč. 11, č. 7 (2021), č. článku 210092. E-ISSN 2046-2441
Institucionální podpora: RVO:67985904
Klíčová slova: DDX21 * p38-MAPK * preimplantation embryo development
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 7.000, rok: 2021
Způsob publikování: Open access
https://royalsocietypublishing.org/doi/10.1098/rsob.210092

Successful navigation of the mouse preimplantation stages of development, during which three distinct blastocyst lineages are derived, represents a prerequisite for continued development. We previously identified a role for p38-mitogen-activated kinases (p38-MAPK) regulating blastocyst inner cell mass (ICM) cell fate, specifically primitive endoderm (PrE) differentiation, that is intimately linked to rRNA precursor processing, polysome formation and protein translation regulation. Here, we develop this work by assaying the role of DEAD-box RNA helicase 21 (DDX21), a known regulator of rRNA processing, in the context of p38-MAPK regulation of preimplantation mouse embryo development. We show nuclear DDX21 protein is robustly expressed from the 16-cell stage, becoming exclusively nucleolar during blastocyst maturation, a localization dependent on active p38-MAPK. siRNA-mediated clonal Ddx21 knockdown within developing embryos is associated with profound cell-autonomous and non-autonomous proliferation defects and reduced blastocyst volume, by the equivalent peri-implantation blastocyst stage. Moreover, ICM residing Ddx21 knockdown clones express the EPI marker NANOG but rarely express the PrE differentiation marker GATA4. These data contribute further significance to the emerging importance of lineage-specific translation regulation, as identified for p38-MAPK, during mouse preimplantation development.
Trvalý link: http://hdl.handle.net/11104/0321905