NAD-capping: a universal RNA modification in Mycobacteria, Archaea and Escherichia coli

0544953 - ÚOCHB 2022 GB eng A - Abstrakt
Ruiz-Larrabeiti, Olatz - Benoni, Roberto - Zemlianski, V. - Hanišáková, N. - Suder, A. - Schwarz, Marek - Brezovská, Barbora - Svojanovská, Barbora - Vítězová, M. - Cahová, Hana - Převorovský, M. - Krásný, Libor
NAD-capping: a universal RNA modification in Mycobacteria, Archaea and Escherichia coli.
FEBS Open Bio. Wiley. Roč. 11, Suppl 1 (2021), s. 18. ISSN 2211-5463. E-ISSN 2211-5463.
[FEBS Congress, Molecules of Life: Towards New Horizons /45./. 03.07.2021-08.07.2021, online]
Institucionální podpora: RVO:61388963 ; RVO:61388971
Obor OECD: Biochemistry and molecular biology
https://doi.org/10.1002/2211-5463.13206

The 50end status is key to the life of a transcript. NAD+(nicoti-namide adenine dinucleotide) universal redox cofactor has beendetected covalently attached to the 5’-end of RNA in bacterial and eukaryotic cells, where RNA polymerase uses NAD+insteadof ATP to initiate transcription. This new RNA modification hasbeen termed NAD-capping. Until now, NAD-capping had notbeen investigated in the mycobacterial genus, which containsimportant pathogens. At the same time, the nature of NAD+cofactor and the discovery of NAD-capping in a widespreadvariety of organisms raised up the possibility that NAD-cappingmay be a universal RNA modification, a hypothesis thatremained unproven until now. Likewise, a cohesive view of thebiological role of NAD-cap and its effect on the transcript andthe cell is still being formed. Here we show that NAD-cappingexists in mycobacteria, and that mycobacterial NAD-RNAs aremRNAs involved in membrane and redox processes and somesmall RNAs (sRNAs), like the characterized Ms1 and rnpB. Wehave also analysed archaeal RNA by mass spectrometry and dis-covered NAD+covalently attached to it, proofing that NAD-cap-ping is universal to all domains of life. Finally, we have studiedthe biological role of NAD-cap in RNAI, the most abundantlymodified sRNA in Escherichia coli. We found that preventingNAD-capping on RNAI caused it to have a longer half-life, andchanged the balance between RNAI and its regulatory target,which affected the ability of the cell to exit LAG phase at highampicillin concentrations, as compared to the wild-type. In thistalk I will explain how our discoveries on NAD-capping inMycobacteria, Archaea and Escherichia coli correlate to previousfindings, and how they add to our knowledge on transcriptomicmodifications.
Trvalý link: http://hdl.handle.net/11104/0321741