Polarization-resolved second-harmonic generation imaging through a multimode fiber

0544708 - ÚPT 2022 RIV US eng J - Článek v odborném periodiku
Cifuentes, Angel S. - Pikálek, Tomáš - Ondráčková, Petra - Amezcua-Correa, R. - Antonio-Lopez, J. E. - Čižmár, Tomáš - Traegaardh, Johanna
Polarization-resolved second-harmonic generation imaging through a multimode fiber.
Optica. Roč. 8, č. 8 (2021), s. 1065-1074. ISSN 2334-2536. E-ISSN 2334-2536
Grant CEP: GA MŠMT EF15_003/0000476; GA MŠMT ED0017/01/01; GA MŠMT(CZ) LO1212
Institucionální podpora: RVO:68081731
Klíčová slova: second harmonic generation * multimode fiber * bioimaging * polarization control
Obor OECD: Optics (including laser optics and quantum optics)
Impakt faktor: 10.644, rok: 2021
Způsob publikování: Open access
https://www.osapublishing.org/optica/fulltext.cfm?uri=optica-8-8-1065&id=453930

Multimode fiber-based endoscopes have recently emerged as a tool for minimally invasive endoscopy in tissue, at depths well beyond the reach of multiphoton imaging. Here, we demonstrate label-free second-harmonic generation (SHG) microscopy through such a fiber endoscope. We simultaneously fully control the excitation polarization state and the spatial distribution of the light at the fiber tip, and we use this to implement polarization-resolved SHG imaging, which allows imaging and identification of structural proteins such as collagen and myosin.We image mouse tail tendon and heart tissue, employing the endoscope at depths up to 1 mm, demonstrating that we can differentiate these structural proteins. This method has the potential for enabling instant and in situ diagnosis of tumors and fibrotic conditions insensitive tissue with minimal damage.
Trvalý link: http://hdl.handle.net/11104/0321538


Vědecká data: Figshare