Knock-Out of ACBD3 Leads to Dispersed Golgi Structure, but Unaffected Mitochondrial Functions in HEK293 and HeLa Cells

0544561 - FGÚ 2022 RIV CH eng J - Článek v odborném periodiku
Daňhelovská, T. - Zdražilová, L. - Štufková, H. - Vanišová, M. - Volfová, N. - Křížová, J. - Kuda, Ondřej - Sládková, J. - Tesařová, M.
Knock-Out of ACBD3 Leads to Dispersed Golgi Structure, but Unaffected Mitochondrial Functions in HEK293 and HeLa Cells.
International Journal of Molecular Sciences. Roč. 22, č. 14 (2021), č. článku 7270. E-ISSN 1422-0067
Grant ostatní: AV ČR(CZ) LQ200111901
Program: Prémie Lumina quaeruntur
Institucionální podpora: RVO:67985823
Klíčová slova: ACBD3 * mitochondria * cholesterol * Golgi * OXPHOS * knock-out
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 6.208, rok: 2021
Způsob publikování: Open access
https://www.mdpi.com/1422-0067/22/14/7270

The Acyl-CoA-binding domain-containing protein (ACBD3) plays multiple roles across the cell. Although generally associated with the Golgi apparatus, it operates also in mitochondria. In steroidogenic cells, ACBD3 is an important part of a multiprotein complex transporting cholesterol into mitochondria. Balance in mitochondrial cholesterol is essential for proper mitochondrial protein biosynthesis, among others. We generated ACBD3 knock-out (ACBD3-KO) HEK293 and HeLa cells and characterized the impact of protein absence on mitochondria, Golgi, and lipid profile. In ACBD3-KO cells, cholesterol level and mitochondrial structure and functions are not altered, demonstrating that an alternative pathway of cholesterol transport into mitochondria exists. However, ACBD3-KO cells exhibit enlarged Golgi area with absence of stacks and ribbon-like formation, confirming the importance of ACBD3 in Golgi stacking. The glycosylation of the LAMP2 glycoprotein was not affected by the altered Golgi structure. Moreover, decreased sphingomyelins together with normal ceramides and sphingomyelin synthase activity reveal the importance of ACBD3 in ceramide transport from ER to Golgi.
Trvalý link: http://hdl.handle.net/11104/0321407