Highly contiguous assemblies of 101 drosophilid genomes

0544518 - BC 2022 RIV GB eng J - Článek v odborném periodiku
Kim, B. Y. - Wang, J. R. - Miller, D. E. - Barmina, O. - Delaney, E. - Thompson, A. - Comeault, A. A. - Peede, D. - D’Agostino, E. R. R. - Pelaez, J. - Aguilar, J. M. - Haji, D. - Matsunaga, T. - Armstrong, E. E. - Zych, M. - Ogawa, Y. - Stamenković-Radak, M. - Jelić, M. - Savić Veselinović, M. - Tanasković, M. - Erić, P. - Gao, J.-J. - Katoh, T. H. - Toda, M. J. - Watabe, H. - Watada, M. - Davis, J. S. - Moyle, L. C. - Manoli, G. - Bertolini, E. - Košťál, Vladimír - Hawley, R. S. - Takahashi, A. - Jones, C. D. - Price, D. K. - Whiteman, N. - Kopp, A. - Matute, D. R. - Petrov, D. A.
Highly contiguous assemblies of 101 drosophilid genomes.
eLife. Roč. 10, JUL 19 (2021), č. článku e66405. ISSN 2050-084X. E-ISSN 2050-084X
Grant CEP: GA ČR(CZ) GA19-13381S
Institucionální podpora: RVO:60077344
Klíčová slova: Drosophilidae * genome * sequencing
Obor OECD: Entomology
Impakt faktor: 8.713, rok: 2021
Způsob publikování: Open access
https://elifesciences.org/articles/66405

Over 100 years of studies in Drosophila melanogaster and related species in the genus Drosophila have facilitated key discoveries in genetics, genomics, and evolution. While high-quality
genome assemblies exist for several species in this group, they only encompass a small fraction of the genus. Recent advances in long-read sequencing allow high-quality genome assemblies for tens or even hundreds of species to be efficiently generated. Here, we utilize Oxford Nanopore sequencing to build an open community resource of genome assemblies for 101 lines of 93 drosophilid species encompassing 14 species groups and 35 sub-groups. The genomes are highly contiguous and complete, with an average contig N50 of 10.5 Mb and greater than 97% BUSCO completeness in 97/101 assemblies. We show that Nanopore-based assemblies are highly accurate in coding regions, particularly with respect to coding insertions and deletions. These assemblies, along with a detailed laboratory protocol and assembly pipelines, are released as a public resource and will serve as a starting point for addressing broad questions of genetics, ecology, and evolution at the scale of hundreds of species.
Trvalý link: http://hdl.handle.net/11104/0327723