Selected ion flow tube mass spectrometry for targeted analysis of volatile organic compounds in human breath

0544250 - ÚFCH JH 2022 RIV GB eng J - Článek v odborném periodiku
Belluomo, I. - Boshier, P. R. - Myridakis, A. - Vadhwana, B. - Markar, S. R. - Španěl, Patrik - Hanna, G. B.
Selected ion flow tube mass spectrometry for targeted analysis of volatile organic compounds in human breath.
Nature Protocols. Roč. 16, č. 7 (2021), s. 3419-3438. ISSN 1754-2189. E-ISSN 1750-2799
Institucionální podpora: RVO:61388955
Klíčová slova: exhaled breath * sift-ms * trace gases * acetone * vapor * lung
Obor OECD: Physical chemistry
Impakt faktor: 17.021, rok: 2021
Způsob publikování: Omezený přístup

The analysis of volatile organic compounds (VOCs) within breath for noninvasive disease detection and monitoring is an emergent research field that has the potential to reshape current clinical practice. However, adoption of breath testing has been limited by a lack of standardization. This protocol provides a comprehensive workflow for online and offline breath analysis using selected ion flow tube mass spectrometry (SIFT-MS). Following the suggested protocol, 50 human breath samples can be analyzed and interpreted in <3 h. Key advantages of SIFT-MS are exploited, including the acquisition of real-time results and direct compound quantification without need for calibration curves. The protocol includes details of methods developed for targeted analysis of disease-specific VOCs, specifically short-chain fatty acids, aldehydes, phenols, alcohols and alkanes. A procedure to make custom breath collection bags is also described. This standardized protocol for VOC analysis using SIFT-MS is intended to provide a basis for wider application and the use of breath analysis in clinical studies.
Trvalý link: http://hdl.handle.net/11104/0321281