Simultaneous use of Interphako interference contrast and polarization microscopy in the study of microorganisms

0544045 - MBÚ 2022 RIV CZ eng J - Článek v odborném periodiku
Žižka, Zdeněk
Simultaneous use of Interphako interference contrast and polarization microscopy in the study of microorganisms.
Folia Microbiologica. Roč. 66, č. 2 (2021), s. 241-246. ISSN 0015-5632. E-ISSN 1874-9356
Grant CEP: GA MŠMT(CZ) LO1509
Institucionální podpora: RVO:61388971
Klíčová slova: Interphako * polarization microscopy * algal cells
Obor OECD: Microbiology
Impakt faktor: 2.629, rok: 2021
Způsob publikování: Omezený přístup
https://link.springer.com/article/10.1007/s12223-020-00839-9

Simultaneous application of polarization microscopy and Interphako interference contrast has been used to study the internal structure of algal cells. The interference contrast technique showed fine cell structures (important is the selection of interference colors according to the Mach-Zehnder interferometer setting). In a polarization microscope, the crossed polarization filters together with the first-order quartz compensator mounted turntable showed the maximum birefringence of the individual structures. Material containing green algae was collected in the villages Sykorice and Zbecno, Protected Landscape Area (PLA) Krivoklatsko. The objects were studied in a Carl Zeiss Jena NfpK laboratory microscope equipped with an In 160 base body with an Interphako In contrast interference module including a Mach-Zehnder interferometer with variable phase contrast, a special condenser with interchangeable aperture plates, a turntable, a Meopta Praha polarizer, a LOMO Sankt Petersburg analyzer, and a quartz compensator with first-order red and the digital camera DSLR Nikon D 70. Green algae of three orders were studied: Siphonocladales, Zygnematales, and Desmidiales. Anisotropic structures were found in all studied representatives of the green algae of the phylum Chlorophyta. Especially their cell walls showed strong birefringence (in all representatives of these orders). On the other hand, a representative of the order Siphonocladales (the genus Cladophora, Cladophoraceae, Ulvophyceae) was rarely found to display weak birefringent granules of storage substances due to the setting of the Mach-Zehnder interferometer and the use of the first-order compensator (interference colors are intensified). In addition, a very weak birefringence of periphyton cells (microbial biofilm) was found.
Trvalý link: http://hdl.handle.net/11104/0321104