Počet záznamů: 1
Oocyte specific lncRNA variant Rose influences oocyte and embryo development
- 1.0543949 - ÚŽFG 2022 RIV CN eng J - Článek v odborném periodiku
Iyyappan, Rajan - Aleshkina, Daria - Zhu, L. - Jiang, Z. - Kinterová, Veronika - Šušor, Andrej
Oocyte specific lncRNA variant Rose influences oocyte and embryo development.
Non-coding RNA research. Roč. 6, č. 2 (2021), s. 107-113. E-ISSN 2468-2160
Grant CEP: GA ČR GA18-19395S
Institucionální podpora: RVO:67985904
Klíčová slova: LncRNA * oocyte * early embryo
Obor OECD: Developmental biology
Způsob publikování: Open access
https://www.sciencedirect.com/science/article/pii/S2468054021000202?via%3Dihub
Fully grown mammalian oocytes store a large amount of RNA synthesized during the transcriptionally active growth stage. A large part of the stored RNA belongs to the long non-coding class which contain either transcriptional noise or important contributors to cellular physiology. Despite the expanding number of studies related to lncRNAs, their influence on oocyte physiology remains enigmatic. We found an oocyte specific antisense, long non-coding RNA, Rose (lncRNA in Oocyte Specifically Expressed) expressed in two variants containing two and three non-coding exons, respectively. Rose is localized in the nucleus of transcriptionally active oocyte and in embryo with polysomal occupancy in the cytoplasm. Experimental overexpression of Rose in fully grown oocyte did not show any differences in meiotic maturation. However, knocking down Rose resulted in abnormalities in oocyte cytokinesis and impaired preimplantation embryo development. In conclusion, we have identified an oocyte-specific maternal lncRNA that is essential for successful mammalian oocyte and embryo development.
Trvalý link: http://hdl.handle.net/11104/0321039
Počet záznamů: 1