Vancomycin-Loaded Collagen/Hydroxyapatite Layers Electrospun on 3D Printed Titanium Implants Prevent Bone Destruction Associated with S. epidermidis Infection and Enhance Osseointegration

0543531 - ÚSMH 2022 RIV CH eng J - Článek v odborném periodiku
Suchý, Tomáš - Vištejnová, L. - Šupová, Monika - Klein, P. - Bartoš, M. - Kolinko, Y. - Blassová, T. - Tonar, Z. - Pokorný, M. - Sucharda, Zbyněk - Žaloudková, Margit - Denk, František - Ballay, R. - Juhás, Štefan - Juhásová, Jana - Klapková, E. - Horný, L. - Sedláček, R. - Grus, T. - Čejka jr., Z. - Čejka, Z. - Chudějová, K. - Hrabák, J.
Vancomycin-Loaded Collagen/Hydroxyapatite Layers Electrospun on 3D Printed Titanium Implants Prevent Bone Destruction Associated with S. epidermidis Infection and Enhance Osseointegration.
Biomedicines. Roč. 9, č. 5 (2021), č. článku 531. E-ISSN 2227-9059
Grant CEP: GA TA ČR(CZ) TA04010330
Institucionální podpora: RVO:67985891 ; RVO:67985904
Klíčová slova: orthopedic implant * collagen * hydroxyapatite * vancomycin * implant-related bone infection * Staphylococcus epidermidis * osseointegration * bone * rat * minipig
Obor OECD: Medical engineering; Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction) (UZFG-Y)
Impakt faktor: 4.757, rok: 2021
Způsob publikování: Open access
https://www.mdpi.com/2227-9059/9/5/531

The aim of the study was to develop an orthopedic implant coating in the form of vancomycin-loaded collagen/hydroxyapatite layers (COLHA+V) that combine the ability to prevent bone infection with the ability to promote enhanced osseointegration. The ability to prevent bone infection was investigated employing a rat model that simulated the clinically relevant implant-related introduction of bacterial contamination to the bone during a surgical procedure using a clinical isolate of Staphylococcus epidermidis. The ability to enhance osseointegration was investigated employing a model of a minipig with terminated growth. Six weeks following implantation, the infected rat femurs treated with the implants without vancomycin (COLHA+S. epidermidis) exhibited the obvious destruction of cortical bone as evinced via a cortical bone porosity of up to 20% greater than that of the infected rat femurs treated with the implants containing vancomycin (COLHA+V+S. epidermidis) (3%) and the non-infected rat femurs (COLHA+V) (2%). The alteration of the bone structure of the infected COLHA+S. epidermidis group was further demonstrated by a 3% decrease in the average Ca/P molar ratio of the bone mineral. Finally, the determination of the concentration of vancomycin released into the blood stream indicated a negligible systemic load. Six months following implantation in the pigs, the quantified ratio of new bone indicated an improvement in osseointegration, with a two-fold bone ingrowth on the COLHA (47%) and COLHA+V (52%) compared to the control implants without a COLHA layer (27%). Therefore, it can be concluded that COLHA+V layers are able to significantly prevent the destruction of bone structure related to bacterial infection with a minimal systemic load and, simultaneously, enhance the rate of osseointegration.
Trvalý link: http://hdl.handle.net/11104/0320826