Počet záznamů: 1
Detailed structural characterization of cardiolipins from various biological sources using a complex analytical strategy comprising fractionation, hydrolysis and chiral chromatography
- 1.0543192 - MBÚ 2022 RIV NL eng J - Článek v odborném periodiku
Vítová, Milada - Stránská, M. - Palyzova, A. - Řezanka, Tomáš
Detailed structural characterization of cardiolipins from various biological sources using a complex analytical strategy comprising fractionation, hydrolysis and chiral chromatography.
Journal of Chromatography A. Roč. 1648, JUL 5 2021 (2021), č. článku 462185. ISSN 0021-9673. E-ISSN 1873-3778
GRANT EU: European Commission(CZ) ATCZ172 - Interreg V-A Austria – Czech Republic
Institucionální podpora: RVO:61388971
Klíčová slova: Enantiomeric separation * Enzymatic hydrolysis * rp-hplc/ms-esi+ * Cardiolipins * Bacterium * Yeast * Alga * Spinach * Bovine heart
Obor OECD: Microbiology
Impakt faktor: 4.601, rok: 2021
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0021967321003095
Cardiolipins (1,3-bis(sn-3'-phosphatidyl)-sn-glycerol) (CLs) are widespread in many organisms, from bacteria to higher green plants and mammals. CLs were observed in Gram-positive bacterium of the genus Kocuria, brewer's yeast Saccharomyces, the green alga Chlamydomonas, spinach and beef heart. A mixture of molecular species of CLs was obtained from total lipids by hydrophilic interaction liquid chromatography (HILIC), and these were further separated and identified by reversed phase LC/MS with negative tandem electrospray ionization. The majority of CLs molecular species from each organism were cleaved using phospholipase C from Bacillus cereus. This phospholipase cleaves CLs into 1,2-diglycerols and phosphatidylglycerol 3-phosphates, which were then separated. After CLs cleavage, diacylglycerols such as sn-1,2-diacyl-3-acetyl-glycerols (i.e., triacylglycerols) were separated and identified by chiral chromatography/MS-positive tandem ESI. Significant differences in the composition of the molecular species between the 3-(3-sn-phosphatidyl) and 1-(3-sn-phosphatidyl) moieties of CLs were found in all organisms tested. Molecular species of CLs that contained four different fatty acids were identified in all five samples, and CLs containing very long chain fatty acids were identified in yeast. In addition, CLs containing both enantiomers (at the sn-2 carbon) were present in the bacterium tested. These findings were further supported by data already published in GenBank where, in the same family Micrococ-caceae both enzymes responsible for chirality in the sn-2 position, glycerol-3-phosphate and glycerol-1-phosphate dehydrogenases, were present. (C) 2021 Elsevier B.V. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0320463
Počet záznamů: 1