Influence of silymarin components on keratinocytes and 3D reconstructed epidermis

0542993 - MBÚ 2022 RIV GB eng J - Článek v odborném periodiku
Franková, J. - Juráňová, J. - Biedermann, David - Ulrichová, J.
Influence of silymarin components on keratinocytes and 3D reconstructed epidermis.
Toxicology in Vitro. Roč. 74, AUG 2021 (2021), č. článku 105162. ISSN 0887-2333. E-ISSN 1879-3177
Grant CEP: GA ČR(CZ) GA18-00132S
Institucionální podpora: RVO:61388971
Klíčová slova: Keratinocytes * rhe * Inflammation * Silymarin
Obor OECD: Toxicology
Impakt faktor: 3.685, rok: 2021
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0887233321000874

Silymarin is a flavonoid complex isolated from the plant Silybum marianum which is well known for its antioxidant, hepatoprotective and immunomodulatory effects. Since little is known about its anti-inflammatory properties and healing effects, our study focused on whether or not silymarin components reduce inflammation and support epidermis regeneration. Lipopolysaccharides (LPS) and sodium dodecyl sulfate (SDS) were used to induce inflammation in normal human epidermal keratinocytes (NHEKs) and reconstructed epidermis (RHE), respectively. The expression of pro-inflammatory cytokines (IL-1, IL-6 and IL-8) in NHEKs and RHE was measured by enzyme linked immunosorbent assay (ELISA). The expression of cytokeratin 14 and loricrin in RHE was detected by immunofluorescent analysis. Hematoxylin and eosin staining was used for the morphological evaluation of RHE. It was determined that 2, 3 dehydrosilybin (DHSB) downregulated the production of selected pro-inflammatory cytokines produced by NHEKs. Although all layers of RHE displayed full thickness, when SDS was applied, cell detachment was seen in the stratum corneum and loricrin expression was diminished.
Trvalý link: http://hdl.handle.net/11104/0320317