0542872 - FGÚ 2022 RIV US eng J - Článek v odborném periodiku
Kolesová, Hana - Olejníčková, Veronika - Kvasilová, A. - Gregorovičová, Martina - Sedmera, David
Tissue clearing and imaging methods for cardiovascular development.
iScience. Roč. 24, č. 4 (2021), č. článku 102387. E-ISSN 2589-0042
Grant CEP: GA MŠMT(CZ) LTC17023; GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) EF16_013/0001775; GA ČR(CZ) GA18-03207S; GA ČR(CZ) GA18-03461S; GA ČR(CZ) GA21-03847S
Institucionální podpora: RVO:67985823
Klíčová slova: tissue clearing * imaging * cardiovascular development
Obor OECD: Cardiac and Cardiovascular systems
Impakt faktor: 6.107, rok: 2021 ; AIS: 1.633, rok: 2021
Způsob publikování: Open access
Web výsledku:
https://doi.org/10.1016/j.isci.2021.102387DOI: https://doi.org/10.1016/j.isci.2021.102387

Tissue imaging in 3D using visible light is limited and various clearing techniques were developed to increase imaging depth, but none provides universal solution for all tissues at all developmental stages. In this review, we focus on different tissue clearing methods for 3D imaging of heart and vasculature, based on chemical composition (solvent-based, simple immersion, hyperhydration, and hydrogel embedding techniques). We discuss in detail compatibility of various tissue clearing techniques with visualization methods: fluorescence preservation, immunohistochemistry, nuclear staining, and fluorescent dyes vascular perfusion. We also discuss myocardium visualization using autofluorescence, tissue shrinking, and expansion. Then we overview imaging methods used to study cardiovascular system and live imaging. We discuss heart and vessels segmentation methods and image analysis. The review covers the whole process of cardiovascular system 3D imaging, starting from tissue clearing and its compatibility with various visualization methods to the types of imaging methods and resulting image analysis.
Trvalý link: http://hdl.handle.net/11104/0320209