Pre- and Neonatal Imprinting on Immunological Homeostasis and Epithelial Barrier Integrity by Escherichia coli Nissle 1917 Prevents Allergic Poly-Sensitization in Mice

0542870 - MBÚ 2022 RIV CH eng J - Článek v odborném periodiku
Sarate, P. J. - Šrůtková, Dagmar - Geissler, N. - Schwarzer, Martin - Schabussova, I. - Inic-Kanada, A. - Kozáková, Hana - Wiedermann, U.
Pre- and Neonatal Imprinting on Immunological Homeostasis and Epithelial Barrier Integrity by Escherichia coli Nissle 1917 Prevents Allergic Poly-Sensitization in Mice.
Frontiers in Immunology. Roč. 11, FEB 17 2021 (2021), č. článku 612775. ISSN 1664-3224. E-ISSN 1664-3224
Grant CEP: GA ČR GA19-02261S; GA MŠMT(CZ) 8J20AT011
Institucionální podpora: RVO:61388971
Klíčová slova: allergic poly-sensitization * germ-free * balb * c * mucosal tolerance * mouse model * Escherichia coli Nissle 1917 * hygiene hypothesis
Obor OECD: Immunology
Impakt faktor: 8.787, rok: 2021
Způsob publikování: Open access
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7927790/

A steady rise in the number of poly-sensitized patients has increased the demand for effective prophylactic strategies against multi-sensitivities. Probiotic bacteria have been successfully used in clinics and experimental models to prevent allergic mono-sensitization. In the present study, we have investigated whether probiotic bacteria could prevent poly-sensitization by imprinting on the immune system early in life. We used two recombinant variants of probiotic Escherichia coli Nissle 1917 (EcN): i) EcN expressing birch and grass pollen, poly-allergen chimera construct (EcN-Chim), and ii) an ´empty´ EcN without allergen expression (EcN-Ctrl). Conventional mice (CV) were treated with either EcN-Chim or EcN-Ctrl in the last week of the gestation and lactation period. Gnotobiotic mice received one oral dose of either EcN-Chim or EcN-Ctrl before mating. The offspring from both models underwent systemic allergic poly-sensitization and intranasal challenge with recombinant birch and grass pollen allergens (rBet v 1, rPhl p 1, and rPhl p 5). In the CV setting, the colonization of offspring via treatment of mothers reduced allergic airway inflammation (AAI) in offspring compared to poly-sensitized controls. Similarly, in a gnotobiotic model, AAI was reduced in EcN-Chim and EcN-Ctrl mono-colonized offspring. However, allergy prevention was more pronounced in the EcN-Ctrl mono-colonized offspring as compared to EcN-Chim. Mono-colonization with EcN-Ctrl was associated with a shift toward mixed Th1/Treg immune responses, increased expression of TLR2 and TLR4 in the lung, and maintained levels of zonulin-1 in lung epithelial cells as compared to GF poly-sensitized and EcN-Chim mono-colonized mice. This study is the first one to establish the model of allergic poly-sensitization in gnotobiotic mice.
Trvalý link: http://hdl.handle.net/11104/0320206