Immune activation by microbiome shapes the colon mucosa: Comparison between healthy rat mucosa under conventional and germ-free conditions

0542580 - MBÚ 2022 RIV GB eng J - Článek v odborném periodiku
Čaja, Fabian - Stakheev, Dmitry - Chernyavskiy, Oleksandr - Křižan, Jiří - Dvořák, Jiří - Rossmann, Pavel - Štěpánková, Renata - Makovický, Peter - Makovický, P. - Kozáková, Hana - Vannucci, Luca
Immune activation by microbiome shapes the colon mucosa: Comparison between healthy rat mucosa under conventional and germ-free conditions.
Journal of Immunotoxicology. Roč. 18, č. 1 (2021), s. 37-49. ISSN 1547-691X. E-ISSN 1547-6901
Grant CEP: GA AV ČR IAA500200917; GA MŠMT(CZ) LM2018126
Výzkumná infrastruktura: CCP II - 90126
Institucionální podpora: RVO:61388971 ; RVO:67985823 ; RVO:68378050
Klíčová slova: Germ-free animals * microbiome * mucosal immunity * collagen * stroma * colon mucosa
Obor OECD: Microbiology; Anatomy and morphology (plant science to be 1.6) (FGU-C)
Impakt faktor: 3.439, rok: 2021
Způsob publikování: Open access
https://www.tandfonline.com/doi/full/10.1080/1547691X.2021.1887412

Germ-free animals (GF) are those without a microbiome since birth. This particular biological model has become one of special interest with the growing evidence of importance of the microbiome in the life, development, adaptation, and immunity of humans and animals in the environments in which they live. Anatomical differences observed in GF compared with conventionally-reared animals (CV) has given rise to the question of the influence of commensal microflora on the development of structure and function (even immunological) of the bowel. Only recently, thanks to achievements in microscopy and associated methods, structural differences can be better evaluated and put in perspective with the immunological characteristics of GF vs. CV animals. This study, using a GF rat model, describes for the first time the possible influence that the presence of commensal microflora, continuously stimulating mucosal immunity, has on the collagen scaffold organization of the colon mucosa. Significant differences were found between CV and GF mucosa structure with higher complexity in the CV rats associated to a more activated immune environment. The immunological data suggest that, in response to the presence of a microbiome, an effective homeostatic regulation in developed by the CV rats in healthy conditions to avoid inflammation and maintain cytokine levels near the spontaneous production found in the GF animals. The results indicated that collagen scaffold adapted to the immune microenvironment, therefore, it is apparent that the microbiome was able to condition the structure of the colon mucosa.
Trvalý link: http://hdl.handle.net/11104/0320539