Počet záznamů: 1
Fluorophore Multimerization as an Efficient Approach towards Bright Protein Labels
- 1.0542326 - ÚOCHB 2022 RIV DE eng J - Článek v odborném periodiku
Reiber, T. - Zavoiura, O. - Dose, C. - Yushchenko, Dmytro A.
Fluorophore Multimerization as an Efficient Approach towards Bright Protein Labels.
European Journal of Organic Chemistry. Roč. 2021, č. 20 (2021), s. 2817-2830. ISSN 1434-193X. E-ISSN 1099-0690
Institucionální podpora: RVO:61388963
Klíčová slova: flow cytometry * fluorescence microscopy * fluorophore * multimers * protein labelling
Obor OECD: Organic chemistry
Impakt faktor: 3.261, rok: 2021
Způsob publikování: Omezený přístup
https://doi.org/10.1002/ejoc.202100117
Cell analysis techniques like flow cytometry and fluorescence microscopy are widely used to explore cells in real‐time and provide important insights into a variety of cellular processes. These techniques require bright and specific staining reagents to generate strong fluorescence signal and enable reliable read‐out, making the choice of fluorescent labels a key aspect of experimental design. Much research has been done in the field of fluorophore development to generate bright small‐molecule dyes, fluorescent proteins, and emitting nanoparticles. However, it remains challenging to modify biomolecules with multiple emitters and avoid self‐quenching of such fluorophores at the same time. Herein, we present advances in multimerization‐based methods for the generation of fluorescent labels with high brightness and discuss their advantages and limitations in the context of flow cytometry and fluorescence microscopy applications.
Trvalý link: http://hdl.handle.net/11104/0319759
Počet záznamů: 1