The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction

0541883 - BFÚ 2022 RIV CH eng J - Článek v odborném periodiku
Legartová, Soňa - Fagherazzi, Paolo - Stixová, Lenka - Kovařík, Aleš - Raška, I. - Bártová, Eva
The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction.
Cells. Roč. 10, č. 2 (2021), č. článku 297. E-ISSN 2073-4409
Grant CEP: GA ČR(CZ) GA18-07384S
Grant ostatní: AV ČR(CZ) StrategieAV21/7
Program: StrategieAV
Institucionální podpora: RVO:68081707
Klíčová slova: splicing * sc-35 * PARP inhibitor * RNA pol II
Obor OECD: Cell biology
Impakt faktor: 7.666, rok: 2021
Způsob publikování: Open access
https://www.mdpi.com/2073-4409/10/2/297

The essential components of splicing are the splicing factors accumulated in nuclear speckles, thus, we studied how DNA damaging agents and A-type lamin depletion affect the properties of these regions, positive on the SC-35 protein. We observed that inhibitor of PARP (poly (ADP-ribose) polymerase), and more pronouncedly inhibitors of RNA polymerases, caused DNA damage and increased the SC-35 protein level. Interestingly, nuclear blebs, induced by PARP inhibitor and observed in A-type lamin-depleted or senescent cells, were positive on both the SC-35 protein and another component of the spliceosome, SRRM2. In the interphase cell nuclei, SC-35 interacted with the phosphorylated form of RNAP II, which was A-type lamin-dependent. In mitotic cells, especially in telophase, the SC-35 protein formed a well-visible ring in the cytoplasmic fraction and colocalized with beta-catenin, associated with the plasma membrane. The antibody against the SRRM2 protein showed that nuclear speckles are already established in the cytoplasm of the late telophase and at the stage of early cytokinesis. In addition, we observed the occurrence of splicing factors in the nuclear blebs and micronuclei, which are also sites of both transcription and splicing. This conclusion supports the fact that splicing proceeds transcriptionally. According to our data, this process is A-type lamin-dependent. Lamin depletion also reduces the interaction between SC-35 and beta-catenin in mitotic cells.
Trvalý link: http://hdl.handle.net/11104/0319380