Temporal Patterns and Intra- and Inter-Cellular Variability in Carbon and Nitrogen Assimilation by the Unicellular Cyanobacterium Cyanothece sp. ATCC 51142

0541527 - MBÚ 2022 RIV CH eng J - Článek v odborném periodiku
Polerecký, L. - Masuda, Takako - Eichner, Meri - Rabouille, S. - Vancová, Marie - Kienhuis, M. V. M. - Bernát, Gábor - Bonomi-Barufi, J. - Campbell, D. A. - Claquin, P. - Červený, Jan - Giordano, Mario - Kotabová, Eva - Kromkamp, J. - Lombardi, A. T. - Lukeš, Martin - Prášil, Ondřej - Stephan, S. - Suggett, D. - Zavřel, Tomáš - Halsey, K. H.
Temporal Patterns and Intra- and Inter-Cellular Variability in Carbon and Nitrogen Assimilation by the Unicellular Cyanobacterium Cyanothece sp. ATCC 51142.
Frontiers in Microbiology. Roč. 12, FEB 4 (2021), č. článku 620915. ISSN 1664-302X. E-ISSN 1664-302X
Grant CEP: GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) EF16_026/0008413; GA ČR(CZ) GA18-24397S; GA ČR(CZ) GA20-17627S; GA ČR(CZ) GJ20-02827Y
Výzkumná infrastruktura: Czech-BioImaging - 90062
Institucionální podpora: RVO:61388971 ; RVO:60077344 ; RVO:86652079
Klíčová slova: Crocosphaera subtropica * Cyanothece * photosynthesis * carbon fixation * nitrogen fixation * nanoSIMS * tem
Obor OECD: Microbiology; Genetics and heredity (medical genetics to be 3) (BC-A); Microbiology (UEK-B)
Impakt faktor: 6.064, rok: 2021
Způsob publikování: Open access
https://www.frontiersin.org/articles/10.3389/fmicb.2021.620915/full

Unicellular nitrogen fixing cyanobacteria (UCYN) are abundant members of phytoplankton communities in a wide range of marine environments, including those with rapidly changing nitrogen (N) concentrations. We hypothesized that differences in N availability (N-2 vs. combined N) would cause UCYN to shift strategies of intracellular N and C allocation. We used transmission electron microscopy and nanoscale secondary ion mass spectrometry imaging to track assimilation and intracellular allocation of C-13-labeled CO2 and N-15-labeled N-2 or NO3 at different periods across a diel cycle in Cyanothece sp. ATCC 51142. We present new ideas on interpreting these imaging data, including the influences of pre-incubation cellular C and N contents and turnover rates of inclusion bodies. Within cultures growing diazotrophically, distinct subpopulations were detected that fixed N-2 at night or in the morning. Additional significant within-population heterogeneity was likely caused by differences in the relative amounts of N assimilated into cyanophycin from sources external and internal to the cells. Whether growing on N-2 or NO3, cells prioritized cyanophycin synthesis when N assimilation rates were highest. N assimilation in cells growing on NO3 switched from cyanophycin synthesis to protein synthesis, suggesting that once a cyanophycin quota is met, it is bypassed in favor of protein synthesis. Growth on NO3 also revealed that at night, there is a very low level of CO2 assimilation into polysaccharides simultaneous with their catabolism for protein synthesis. This study revealed multiple, detailed mechanisms underlying C and N management in Cyanothece that facilitate its success in dynamic aquatic environments.
Trvalý link: http://hdl.handle.net/11104/0319085