Počet záznamů: 1  

Comparative Omics Analysis of Historic and Recent Isolates of Bordetella pertussis and Effects of Genome Rearrangements on Evolution

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    0541481 - MBÚ 2022 RIV US eng J - Článek v odborném periodiku
    Dienstbier, Ana - Amman, F. - Petráčková, Denisa - Štipl, Daniel - Čapek, Jan - Zavadilová, J. - Fabiánová, K. - Držmíšek, Jakub - Kumar, Dilip - Wildung, M. - Pouchnik, D. - Večerek, Branislav
    Comparative Omics Analysis of Historic and Recent Isolates of Bordetella pertussis and Effects of Genome Rearrangements on Evolution.
    Emerging Infectious Diseases. Roč. 27, č. 1 (2021), s. 57-68. ISSN 1080-6040. E-ISSN 1080-6059
    Grant CEP: GA MZd(CZ) NV16-30782A; GA ČR(CZ) GA19-12338S; GA MŠMT(CZ) ED1.1.00/02.0109
    Grant ostatní: AV ČR(CZ) MSM200201702
    Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
    Institucionální podpora: RVO:61388971
    Klíčová slova: secretion system * gene * vaccine * phosphoglucomutase * lipopolysaccharide * expression * virulence * alignment
    Obor OECD: Microbiology
    Impakt faktor: 16.126, rok: 2021
    Způsob publikování: Open access
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7774529/

    Despite high vaccination coverage, pertussis is increasing in many industrialized countries, including the Czech Republic. To better understand Bordetella pertussis resurgence, we analyzed historic strains and recent clinical isolates by using a comparative omics approach. Whole-genome sequencing showed that historic and recent isolates of B. pertussis have substantial variation in genome organization and form separate phylogenetic clusters. Subsequent RNA sequence analysis and liquid chromatography with mass tandem spectrometry analyses showed that these variations translated into discretely separated transcriptomic and proteomic profiles. When compared with historic strains, recent isolates showed increased expression of flagellar genes and genes involved in lipopolysaccharide biosynthesis and decreased expression of polysaccharide capsule genes. Compared with reference strain Tohama I, all strains had increased expression and production of the type III secretion system apparatus. We detected the potential link between observed effects and insertion sequence element-induced changes in gene context only for a few genes.
    Trvalý link: http://hdl.handle.net/11104/0319030

     
     
Počet záznamů: 1  

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