Počet záznamů: 1
Molecular cytogenetic analysis of a triploid population of the human broad tapeworm, Dibothriocephalus latus (Diphyllobothriidea)
- 1.0541478 - BC 2022 RIV US eng J - Článek v odborném periodiku
Orosová, M. - Marková, A. - Provazníková, Irena - Oros, M. - Radačovská, A. - Čadková, Z. - Marec, František
Molecular cytogenetic analysis of a triploid population of the human broad tapeworm, Dibothriocephalus latus (Diphyllobothriidea).
Parasitology. Roč. 148, č. 7 (2021), s. 787-797. ISSN 0031-1820. E-ISSN 1469-8161
Grant ostatní: AV ČR(CZ) SAV-18-20
Program: Bilaterální spolupráce
Institucionální podpora: RVO:60077344
Klíčová slova: 18S rDNA * parthenogenesis * polyploidization
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 3.243, rok: 2021
Způsob publikování: Omezený přístup
https://www.cambridge.org/core/journals/parasitology/article/abs/molecular-cytogenetic-analysis-of-a-triploid-population-of-the-human-broad-tapeworm-dibothriocephalus-latus-diphyllobothriidea/8D9DE3C57EA75A303B8099EEAA202AA8
The large-sized tapeworm Dibothriocephalus latus is known as the broad or fish-borne cestode of mammals that is capable to infect humans and cause diphyllobothriosis. Recently, molecular data on D. latus has been accumulating in the literature and a complete genome sequence has been published, however, little is known about the karyotype and chromosome architecture. In this study, an in-depth karyological analysis of 2 D. latus specimens was carried out. The plerocercoids originated from a perch caught in subalpine Lake Iseo (Italy) and the tapeworms were reared in hamsters. Both specimens contained cells with a highly variable number of chromosomes ranging from18 to 27. Nevertheless, the largest portion of mitotic figures (47%) showed a number corresponding to the triploid set, 3n = 27. Accordingly, the karyotype of the analyzed specimens consisted of 9 triplets of metacentric chromosomes. Fluorescence in situ hybridization (FISH) with the 18S rDNA probe clearly demonstrated the presence of 3 clusters of hybridization signals on the triplet of chromosome 7, thus confirming the triploid status of the specimens. FISH with a telomeric (TTAGGG)n probe confined hybridization signals exclusively to the terminal chromosomal regions, supporting the earlier findings that this
repetitive motif is a conserved feature of tapeworm telomeres.
Trvalý link: http://hdl.handle.net/11104/0326392
Počet záznamů: 1