Structural basis of prostate-specific membrane antigen recognition by the A9g RNA aptamer

0541410 - BTÚ 2021 RIV GB eng J - Článek v odborném periodiku
Ptáček, Jakub - Zhang, D. - Qiu, L. - Kruspe, S. - Motlová, Lucia - Kolenko, Petr - Nováková, Zora - Shubham, S. - Havlínová, Barbora - Baranová, Petra - Chen, S.-J. - Zou, X. - Giangrande, P. - Bařinka, Cyril
Structural basis of prostate-specific membrane antigen recognition by the A9g RNA aptamer.
Nucleic Acids Research. Roč. 48, č. 19 (2020), s. 11130-11145. ISSN 0305-1048. E-ISSN 1362-4962
Grant CEP: GA ČR(CZ) GA18-04790S; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) LTAUSA18196
Institucionální podpora: RVO:86652036
Klíčová slova: GLUTAMATE-CARBOXYPEPTIDASE-II * MONOCLONAL-ANTIBODIES * CRYSTAL-STRUCTURE
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 16.971, rok: 2020
Způsob publikování: Open access
https://academic.oup.com/nar/article/48/19/11130/5856120

Prostate-specific membrane antigen (PSMA) is a well-characterized tumor marker associated with prostate cancer and neovasculature of most solid tumors. PSMA-specific ligands are thus being developed to deliver imaging or therapeutic agents to cancer cells. Here, we report on a crystal structure of human PSMA in complex with A9g, a 43-bp PSMA-specific RNA aptamer, that was determined to the 2.2 angstrom resolution limit. The analysis of the PSMA/aptamer interface allows for identification of key interactions critical for nanomolar binding affinity and high selectivity of A9g for human PSMA. Combined with in silico modeling, site-directed mutagenesis, inhibition experiments and cell-based assays, the structure also provides an insight into structural changes of the aptamer and PSMA upon complex formation, mechanistic explanation for inhibition of the PSMA enzymatic activity by A9g as well as its ligand-selective competition with small molecules targeting the internal pocket of the enzyme. Additionally, comparison with published protein-RNA aptamer structures pointed toward more general features governing protein-aptamer interactions. Finally, our findings can be exploited for the structure-assisted design of future A9g-based derivatives with improved binding and stability characteristics.
Trvalý link: http://hdl.handle.net/11104/0318975