Evaluation of pulsed electric fields technology for the improvement of subsequent carotenoid extraction from dried Rhodotorula glutinis yeast.

0541354 - ÚCHP 2022 RIV NL eng J - Článek v odborném periodiku
Martínez, J.M. - Schottroff, F. - Haas, K. - Fauster, T. - Sajfrtová, Marie - Álvarez, I. - Raso, J. - Jaeger, H.
Evaluation of pulsed electric fields technology for the improvement of subsequent carotenoid extraction from dried Rhodotorula glutinis yeast.
Food Chemistry. Roč. 323, SEP 1 (2020), č. článku 126824. ISSN 0308-8146. E-ISSN 1873-7072
Institucionální podpora: RVO:67985858
Klíčová slova: carotenoids * supercritical fluids * extraction
Obor OECD: Chemical process engineering
Impakt faktor: 7.514, rok: 2020
Způsob publikování: Open access s časovým embargem

This research aims to evaluate whether the electroporation of Rhodotorula glutinis fresh biomass improved the subsequent extraction of carotenoids from dry biomass using supercritical CO2 and traditional solvent extraction.
Supercritical CO2 extraction yields were low after all treatments assayed. Similarly, solvent extraction of carotenoids from untreated or PEF treated cells that were immediately freeze-dried after the pre-treatment was neither effective (extraction yield < 20% total content).
Conversely, PEF-treatment and subsequent intermediate incubation in aqueous buffer for 24 h, followed by freeze-drying and extraction, led to a large improvement with the three solvents assayed (acetone, hexane, ethanol). Ethanol was the most efficient, reaching an extraction yield of 80% of total carotenoid, which represents a recovery of 267 mu g/g(dw). Torularhodin esters constituted the main carotenoid found in the extracts. This is of great interest, as ethanol is eco-friendly solvent and potential applications of torularhodin range from food to medical purposes.



Trvalý link: http://hdl.handle.net/11104/0318916