Počet záznamů: 1  

Proteomic Characterization of Human Neural Stem Cells and Their Secretome During in vitro Differentiation

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    0541278 - ÚŽFG 2022 RIV CH eng J - Článek v odborném periodiku
    Červenka, Jakub - Tylečková, Jiřina - Kupcová Skalníková, Helena - Vodičková Kepková, Kateřina - Poliakh, Ievgeniia - Valeková, Ivona - Pfeiferová, Lucie - Kolář, Michal - Vaškovičová, Michaela - Pánková, Tereza - Vodička, Petr
    Proteomic Characterization of Human Neural Stem Cells and Their Secretome During in vitro Differentiation.
    Frontiers in Cellular Neuroscience. Roč. 14, JAN 28 (2021), č. článku 612560. E-ISSN 1662-5102
    Grant CEP: GA MŠMT(CZ) LTC18079; GA MŠMT(CZ) LO1609; GA MŠMT(CZ) EF16_019/0000785
    Institucionální podpora: RVO:67985904 ; RVO:68378050
    Klíčová slova: neural stem cells * proliferation * neural differentiation
    Obor OECD: Biochemistry and molecular biology
    Impakt faktor: 6.147, rok: 2021
    Způsob publikování: Open access
    https://www.frontiersin.org/articles/10.3389/fncel.2020.612560/full

    Cell therapies represent a promising approach to slow down the progression of currently untreatable neurodegenerative diseases (e.g., Alzheimer's and Parkinson's disease or amyotrophic lateral sclerosis), as well as to support the reconstruction of functional neural circuits after spinal cord injuries. In such therapies, the grafted cells could either functionally integrate into the damaged tissue, partially replacing dead or damaged cells, modulate inflammatory reaction, reduce tissue damage, or support neuronal survival by secretion of cytokines, growth, and trophic factors. Comprehensive characterization of cells and their proliferative potential, differentiation status, and population purity before transplantation is crucial to preventing safety risks, e.g., a tumorous growth due to the proliferation of undifferentiated stem cells. We characterized changes in the proteome and secretome of human neural stem cells (NSCs) during their spontaneous (EGF/FGF2 withdrawal) differentiation and differentiation with trophic support by BDNF/GDNF supplementation. We used LC-MS/MS in SWATH-MS mode for global cellular proteome profiling and quantified almost three thousand cellular proteins. Our analysis identified substantial protein differences in the early stages of NSC differentiation with more than a third of all the proteins regulated (including known neuronal and NSC multipotency markers) and revealed that the BDNF/GDNF support affected more the later stages of the NSC differentiation. Among the pathways identified as activated during both spontaneous and BDNF/GDNF differentiation were the HIF-1 signaling pathway, Wnt signaling pathway, and VEGF signaling pathway. Our follow-up secretome analysis using Luminex multiplex immunoassay revealed significant changes in the secretion of VEGF and IL-6 during NSC differentiation. Our results further demonstrated an increased expression of neuropilin-1 as well as catenin beta-1, both known to participate in the regulation of VEGF signaling, and showed that VEGF-A isoform 121 (VEGF121), in particular, induces proliferation and supports survival of differentiating cells.
    Trvalý link: http://hdl.handle.net/11104/0318862

     
     
Počet záznamů: 1  

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