Adenosine receptor ant its downstream targets, Mod(mdg4) and Hsp70, work as a signaling pathway modulating cytotoxic damage in Drosophila

0541060 - BC 2022 RIV CH eng J - Článek v odborném periodiku
Lin, Yu-Hsien - Maaroufi, Houda Ouns - Kučerová, Lucie - Rouhová, Lenka - Filip, Tomáš - Žurovec, Michal
Adenosine receptor ant its downstream targets, Mod(mdg4) and Hsp70, work as a signaling pathway modulating cytotoxic damage in Drosophila.
Frontiers in Cell and Developmental Biology. Roč. 9, MAR 12 (2021), č. článku 651367. ISSN 2296-634X. E-ISSN 2296-634X
Grant CEP: GA ČR(CZ) GJ19-13784Y
Grant ostatní: GA JU(CZ) 065/2017/P; Program Interreg(CZ) REGGEN/ATCZ207
Institucionální podpora: RVO:60077344
Klíčová slova: heat-shock protein 70 * modifier of mdg4 * mutant huntingtin
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 6.081, rok: 2021
Způsob publikování: Open access
https://www.frontiersin.org/articles/10.3389/fcell.2021.651367/pdf

Adenosine (Ado) is an important signaling molecule involved in stress responses. Studies in mammalian models have shown that Ado regulates signaling mechanisms involved in ‘danger-sensing’ and tissue-protection. Yet, little is known about the role of Ado signaling in Drosophila. In the present study, we observed lower extracellular Ado concentration and suppressed expression of Ado transporters in flies expressing mutant huntingtin protein (mHTT). We altered Ado signaling using genetic tools and found that the overexpression of Ado metabolic enzymes, as well as the suppression of Ado receptor (AdoR) and transporters (ENTs), were able to minimize mHTT-induced mortality. We also identified the downstream targets of the AdoR pathway, the modifier of mdg4 (Mod(mdg4)) and heat-shock protein 70 (Hsp70), which carry out its function. Finally, we showed that a decrease in Ado signaling affect other Drosophila stress reactions, including paraquat and heat-shock treatments. Our study provides important insights into how Ado regulates stress responses in Drosophila.
Trvalý link: http://hdl.handle.net/11104/0326500