Trypanosomatids

0540959 - BC 2021 RIV US eng M - Část monografie knihy
Lukeš, Julius - Votýpka, Jan
Field isolation and cultivation of trypanosomatids from insects.
Trypanosomatids. New York: Humana, 2020 - (Michels, P.; Ginger, M.; Zilberstein, D.), s. 3-21. Methods in Molecular Biology. ISBN 978-1-0716-0294-2
Grant CEP: GA ČR(CZ) GA18-15962S; GA MŠMT(CZ) EF16_019/0000759; GA MŠMT(CZ) LL1601
Institucionální podpora: RVO:60077344
Klíčová slova: iCLAP * iCLIP * Posttranscriptional gene regulation * RNA-binding proteins (RBPs)
Obor OECD: Biochemistry and molecular biology
https://link.springer.com/protocol/10.1007%2F978-1-0716-0294-2_1

RNA-binding proteins (RBPs) are critical to posttranscriptional gene regulation. Therefore, characterization of the RNA molecules bound by RBPs in vivo represent a key step in elucidating their function. The recently developed iCLIP technique allows single nucleotide resolution of the RNA binding footprints of RBPs. We present the iCLIP technique modified for its application to Trypanosoma brucei and most likely other kinetoplastid flagellates. By using the immuno- or affinity purification approach, it was successfully applied to the analysis of several RBPs. Furthermore, we also provide a detailed description of the iCLIP/iCLAP protocol that shall be particularly suitable for the studies of trypanosome RBPs.
Trvalý link: http://hdl.handle.net/11104/0318543