Počet záznamů: 1  

Gold nanoparticles coated with polyvinylpyrrolidone and sea urchin extracellular molecules induce transient immune activation

  1. 1.
    0540711 - MBÚ 2021 RIV NL eng J - Článek v odborném periodiku
    Alijagic, A. - Barbero, F. - Gaglio, D. - Napodano, E. - Benada, Oldřich - Kofroňová, Olga - Puntes, V. F. - Bastús, N. G. - Pinsino, A.
    Gold nanoparticles coated with polyvinylpyrrolidone and sea urchin extracellular molecules induce transient immune activation.
    Journal of Hazardous Materials. Roč. 402, JAN 15 (2021), č. článku 123793. ISSN 0304-3894. E-ISSN 1873-3336
    Grant CEP: GA MŠMT(CZ) LO1509
    GRANT EU: European Commission(XE) 671881 - PANDORA
    Institucionální podpora: RVO:61388971
    Klíčová slova: Sea urchin immune cells * Innate defence response * Nano-recognition * Immune metabolic rewiring * Immunoreactivity
    Obor OECD: Immunology
    Impakt faktor: 14.224, rok: 2021
    Způsob publikování: Omezený přístup
    https://www.sciencedirect.com/science/article/abs/pii/S0304389420317829

    We report that the immunogenicity of colloidal gold nanoparticles coated with polyvinylpyrrolidone (PVP-AuNPs) in a model organism, the sea urchin Paracentrotus lividus, can function as a proxy for humans for in vitro immunological studies. To profile the immune recognition and interaction from exposure to PVP-AuNPs (1 and 10 mu g mL-1), we applied an extensive nano-scale approach, including particle physicochemical character-isation involving immunology, cellular biology, and metabolomics. The interaction between PVP-AuNPs and soluble proteins of the sea urchin physiological coelomic fluid (blood equivalent) results in the formation of a protein ´corona´ surrounding the NPs from three major proteins that influence the hydrodynamic size and colloidal stability of the particle. At the lower concentration of PVP-AuNPs, the P. lividus phagocytes show a broad metabolic plasticity based on the biosynthesis of metabolites mediating inflammation and phagocytosis. At the higher concentration of PVP-AuNPs, phagocytes activate an immunological response involving Toll-like receptor 4 (TLR4) signalling pathway at 24 hours of exposure. These results emphasise that exposure to PVP-AuNPs drives inflammatory signalling by the phagocytes and the resolution at both the low and high concentrations of the PVP-AuNPs and provides more details regarding the immunogenicity of these NPs.
    Trvalý link: http://hdl.handle.net/11104/0318328

     
     
Počet záznamů: 1  

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