Počet záznamů: 1
Gold nanoparticles coated with polyvinylpyrrolidone and sea urchin extracellular molecules induce transient immune activation
- 1.0540711 - MBÚ 2021 RIV NL eng J - Článek v odborném periodiku
Alijagic, A. - Barbero, F. - Gaglio, D. - Napodano, E. - Benada, Oldřich - Kofroňová, Olga - Puntes, V. F. - Bastús, N. G. - Pinsino, A.
Gold nanoparticles coated with polyvinylpyrrolidone and sea urchin extracellular molecules induce transient immune activation.
Journal of Hazardous Materials. Roč. 402, JAN 15 (2021), č. článku 123793. ISSN 0304-3894. E-ISSN 1873-3336
Grant CEP: GA MŠMT(CZ) LO1509
GRANT EU: European Commission(XE) 671881 - PANDORA
Institucionální podpora: RVO:61388971
Klíčová slova: Sea urchin immune cells * Innate defence response * Nano-recognition * Immune metabolic rewiring * Immunoreactivity
Obor OECD: Immunology
Impakt faktor: 14.224, rok: 2021
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/abs/pii/S0304389420317829
We report that the immunogenicity of colloidal gold nanoparticles coated with polyvinylpyrrolidone (PVP-AuNPs) in a model organism, the sea urchin Paracentrotus lividus, can function as a proxy for humans for in vitro immunological studies. To profile the immune recognition and interaction from exposure to PVP-AuNPs (1 and 10 mu g mL-1), we applied an extensive nano-scale approach, including particle physicochemical character-isation involving immunology, cellular biology, and metabolomics. The interaction between PVP-AuNPs and soluble proteins of the sea urchin physiological coelomic fluid (blood equivalent) results in the formation of a protein ´corona´ surrounding the NPs from three major proteins that influence the hydrodynamic size and colloidal stability of the particle. At the lower concentration of PVP-AuNPs, the P. lividus phagocytes show a broad metabolic plasticity based on the biosynthesis of metabolites mediating inflammation and phagocytosis. At the higher concentration of PVP-AuNPs, phagocytes activate an immunological response involving Toll-like receptor 4 (TLR4) signalling pathway at 24 hours of exposure. These results emphasise that exposure to PVP-AuNPs drives inflammatory signalling by the phagocytes and the resolution at both the low and high concentrations of the PVP-AuNPs and provides more details regarding the immunogenicity of these NPs.
Trvalý link: http://hdl.handle.net/11104/0318328
Počet záznamů: 1