Počet záznamů: 1  

ADAMTS Proteases: Methods and Protocols

  1. 1.
    0540393 - ÚEM 2021 US eng M - Část monografie knihy
    Souter, L. - Kwok, Jessica
    Visualization of Perineuronal Nets in Central Nervous System Tissue Sections.
    ADAMTS Proteases: Methods and Protocols. HUMANA PRESS INC, 2020 - (Apte, S.), s. 251-260. ISBN 978-1-4939-9698-8; 978-1-4939-9697-1
    Grant CEP: GA MŠMT(CZ) EF15_003/0000419
    Institucionální podpora: RVO:68378041
    Klíčová slova: perineuronal nets * wisteria floribunda agglutinin * extracellular-matrix
    Obor OECD: Neurosciences (including psychophysiology

    The perineuronal net (PNN) is a specialized extracellular matrix structure that surrounds subpopulations of neurons in the central nervous system (CNS). The appearance of PNNs on the cell surface marks the closure of the critical period during development and has been observed to reduce synaptic plasticity. Perineuronal nets comprise hyaluronan, chondroitin sulfate proteoglycans (CSPGs), link proteins, tenascin-R, and other components, some of which are substrates for a disintegrin-like and metalloprotease domain with thrombospondin type 1 motifs (ADAMTS) proteases. There is a high heterogeneity of PNNs in the CNS. Depending on which part of the CNS is studied, the PNNs may be observed surrounding the soma, or both the soma and proximal dendrites. The most robust marker for PNN is a lectin called Wisteria floribunda agglutinin. Here, we describe a method for preparing tissue for visualization of PNNs in CNS.
    Trvalý link: http://hdl.handle.net/11104/0318015

     
     
Počet záznamů: 1  

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