Immuno-probed graphene nanoplatelets on electrolyte-gated field-effect transistor for stable cortisol quantification in serum

0540097 - FZÚ 2021 RIV NL eng J - Článek v odborném periodiku
Nasyifa, M.M.N. - Ruslinda, A.R. - Halim, N.H.A. - Abidin, A.S.Z. - Faudzi, F.N.M. - Ahmad, N.A. - Lockman, Z. - Rezek, B. - Kromka, Alexander - Gopinath, S.Ch.B.
Immuno-probed graphene nanoplatelets on electrolyte-gated field-effect transistor for stable cortisol quantification in serum.
Journal of the Taiwan Institute of Chemical Engineers. Roč. 117, Dec (2020), s. 10-18. ISSN 1876-1070. E-ISSN 1876-1089
Grant CEP: GA MŠMT(CZ) EF16_019/0000760
Grant ostatní: OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
Institucionální podpora: RVO:68378271
Klíčová slova: immunosensor * field-effect transistor * graphene nanoplatelets * cortisol * human serum
Obor OECD: Food and beverages
Impakt faktor: 5.876, rok: 2020
Způsob publikování: Omezený přístup
https://doi.org/10.1016/j.jtice.2020.12.008

This study reports immuno-probed graphene nanoplatelets on electrolyte-gated field-effect transistor (EGFET) biosensor for cortisol determination in human serum. Solution-processed graphene nanoplatelets were evidenced by Raman spectroscopy and utilized as the transducing element on the FET. Further, confirmed the binding events of the antibody on graphene nanoplatelets using XPS and characterized the electrostatic gating effect of cortisol and intermediate functionalization on graphene nanoplatelets-EGFET. The biosensor exhibited good sensitivity of 72.30 uA.(g/mL)-1 in a linear range between 1.00 pg/mL to 10.00 ng/mL, with a limit of detection (LOD) of 0.85 pg/mL. Cortisol was also successfully detected with interference by the human serum, suggesting the capability of graphene nanoplatelets-EGFET sensor for determining cortisol in a complex matrix.
Trvalý link: http://hdl.handle.net/11104/0317756