Počet záznamů: 1  

Catalogue of stage-specific transcripts inIxodes ricinusand their potential functions during the tick life-cycle

  1. 1.
    0539355 - BFÚ 2021 RIV GB eng J - Článek v odborném periodiku
    Věchtová, Pavlína - Füssy, Z. - Čegan, Radim - Štěrba, Ján - Erhart, Jan - Benes, V. - Grubhoffer, Libor
    Catalogue of stage-specific transcripts inIxodes ricinusand their potential functions during the tick life-cycle.
    Parasites & Vectors. Roč. 13, č. 1 (2020), č. článku 311. ISSN 1756-3305. E-ISSN 1756-3305
    Grant CEP: GA ČR(CZ) GA18-27204S; GA MŠMT(CZ) LTARF18021; GA MŠMT(CZ) EF16_019/0000759
    Institucionální podpora: RVO:68081707 ; RVO:60077344
    Klíčová slova: rhipicephalus boophilus microplus * borne encephalitis tbe * gene-expression * hard tick * ornithodoros-moubata * aegypti mosquitos * salivary-glands
    Obor OECD: Parasitology; Virology (BC-A)
    Impakt faktor: 3.876, rok: 2020
    Způsob publikování: Open access
    https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04173-4

    Background The castor bean tickIxodes ricinusis an important vector of several clinically important diseases, whose prevalence increases with accelerating global climate changes. Characterization of a tick life-cycle is thus of great importance. However, researchers mainly focus on specific organs of fed life stages, while early development of this tick species is largely neglected. Methods In an attempt to better understand the life-cycle of this widespread arthropod parasite, we sequenced the transcriptomes of four life stages (egg, larva, nymph and adult female), including unfed and partially blood-fed individuals. To enable a more reliable identification of transcripts and their comparison in all five transcriptome libraries, we validated an improved-fit set of fiveI. ricinus-specific reference genes for internal standard normalization of our transcriptomes. Then, we mapped biological functions to transcripts identified in different life stages (clusters) to elucidate life stage-specific processes. Finally, we drew conclusions from the functional enrichment of these clusters specifically assigned to each transcriptome, also in the context of recently published transcriptomic studies in ticks. Results We found that reproduction-related transcripts are present in both fed nymphs and fed females, underlining the poorly documented importance of ovaries as moulting regulators in ticks. Additionally, we identified transposase transcripts in tick eggs suggesting elevated transposition during embryogenesis, co-activated with factors driving developmental regulation of gene expression. Our findings also highlight the importance of the regulation of energetic metabolism in tick eggs during embryonic development and glutamate metabolism in nymphs. Conclusions Our study presents novel insights into stage-specific transcriptomes ofI. ricinusand extends the current knowledge of this medically important pathogen, especially in the early phases of its development.
    Trvalý link: http://hdl.handle.net/11104/0317044

     
     
Počet záznamů: 1  

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