In-Depth Bioinformatic Analyses ofNidoviralesIncluding Human SARS-CoV-2, SARS-CoV, MERS-CoV Viruses Suggest Important Roles of Non-canonical Nucleic Acid Structures in Their Lifecycles

0539333 - BFÚ 2021 RIV CH eng J - Článek v odborném periodiku
Bartas, M. - Brázda, Václav - Bohalova, Natalia - Cantara, Alessio - Volna, A. - Stachurová, T. - Malachová, K. - Jagelská, Eva - Porubiakova, Otilia - Cerven, J. - Pečinka, P.
In-Depth Bioinformatic Analyses ofNidoviralesIncluding Human SARS-CoV-2, SARS-CoV, MERS-CoV Viruses Suggest Important Roles of Non-canonical Nucleic Acid Structures in Their Lifecycles.
Frontiers in Microbiology. Roč. 11, jul 3 2020 (2020), č. článku 1583. ISSN 1664-302X. E-ISSN 1664-302X
Institucionální podpora: RVO:68081707
Klíčová slova: g-quadruplex structures * web-based server * molecular-biology * rna * dna
Obor OECD: Microbiology
Impakt faktor: 5.640, rok: 2020
Způsob publikování: Open access
https://www.frontiersin.org/articles/10.3389/fmicb.2020.01583/full

Non-canonical nucleic acid structures play important roles in the regulation of molecular processes. Considering the importance of the ongoing coronavirus crisis, we decided to evaluate genomes of all coronaviruses sequenced to date (stated more broadly, the orderNidovirales) to determine if they contain non-canonical nucleic acid structures. We discovered much evidence of putative G-quadruplex sites and even much more of inverted repeats (IRs) loci, which in fact are ubiquitous along the whole genomic sequence and indicate a possible mechanism for genomic RNA packaging. The most notable enrichment of IRs was found inside 5 ' UTR for IRs of size 12+ nucleotides, and the most notable enrichment of putative quadruplex sites (PQSs) was located before 3 ' UTR, inside 5 ' UTR, and before mRNA. This indicates crucial regulatory roles for both IRs and PQSs. Moreover, we found multiple G-quadruplex binding motifs in human proteins having potential for binding of SARS-CoV-2 RNA. Non-canonical nucleic acids structures inNidoviralesand in novel SARS-CoV-2 are therefore promising druggable structures that can be targeted and utilized in the future.
Trvalý link: http://hdl.handle.net/11104/0317023