Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

0539261 - ÚEM 2021 RIV GB eng J - Článek v odborném periodiku
Moller, P. - Azqueta, A. - Boutet-Robinet, E. - Koppen, G. - Bonassi, S. - Milić, M. - Gajski, G. - Costa, S. - Teixeira, J. P. - Costa Pereira, C. - Dusinska, M. - Godschalk, R. - Brunborg, G. - Gützkow, K.B. - Giovannelli, L. - Cooke, M.S. - Richling, E. - Laffon, B. - Valdiglesias, V. - Basaran, N. - Del Bo', C. - Zegura, B. - Novák, M. - Stopper, H. - Vodička, Pavel - Vodenková, Soňa - de Andrade, V.M. - Sramkova, M. - Gábelová, A. - Collins, A. - Langie, S.A.S.
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results.
Nature Protocols. Roč. 15, č. 12 (2020), s. 3817-3826. ISSN 1754-2189. E-ISSN 1750-2799
Institucionální podpora: RVO:68378041
Klíčová slova: inter-laboratory variation * DNA-damage * animal-models
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 13.491, rok: 2020
Způsob publikování: Open access
https://www.nature.com/articles/s41596-020-0398-1

The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between desirable and essential information: essential information refers to the precise details that are necessary to assess the quality of the experimental work, whereas desirable information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.

Here, members of the hCOMET COST Action program provide a consensus statement on the Minimum Information for Reporting Comet Assays (MIRCA).
Trvalý link: http://hdl.handle.net/11104/0316930