Trigeminal neuropathic pain is alleviated by inhibition of Cav3.3 T-type calcium channels in mice

0539125 - ÚOCHB 2022 RIV US eng J - Článek v odborném periodiku
Montera, M. - Goins, A. - Cmarko, Leoš - Weiss, Norbert - Westlund, K. N. - Alles, S. R. A.
Trigeminal neuropathic pain is alleviated by inhibition of Cav3.3 T-type calcium channels in mice.
Channels. Roč. 15, č. 1 (2021), s. 31-37. ISSN 1933-6950. E-ISSN 1933-6969
Institucionální podpora: RVO:61388963
Klíčová slova: neuropathic pain * calcium channels * Cav3.3 * trigeminal nerve injury * therapeutics
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 3.493, rok: 2021
Způsob publikování: Open access
https://doi.org/10.1080/19336950.2020.1859248

In this brief report, we demonstrate that the Cav3.3 T-type voltage-gated calcium channel subtype is involved in our FRICT-ION model of chronic trigeminal neuropathic pain. We first showed that the Cacna1i gene encoding Cav3.3 is significantly upregulated in whole trigeminal ganglia of FRICT-ION mice compared to controls at week 10 post-injury. We confirmed protein upregulation of Cav3.3 compared to controls using Western blot analysis of whole trigeminal ganglia tissues. Finally, we demonstrated that intraperitoneal injection of a selective TAT-based Cav3.3 blocking peptide in FRICT-ION mice significantly reduces Cav3.3 protein expression at the peak anti-allodynic effect (4 hrs post-injection) of the attenuated neuropathic pain behavior. We also suggest that blockade of Cav3.3 may be more effective in attenuating trigeminal neuropathic pain in female than male FRICT-ION mice. Therefore, blocking or attenuating Cav3.3 function may be an effective strategy for the treatment of trigeminal neuropathic pain.
Trvalý link: http://hdl.handle.net/11104/0317086