Cholinergic axons regulate type I acini in salivary glands of Ixodes ricinus and Ixodes scapularis ticks

0538699 - BC 2021 RIV GB eng J - Článek v odborném periodiku
Mateos-Hernandez, L. - Defaye, B. - Vancová, Marie - Hajdušek, Ondřej - Šíma, Radek - Park, Y. - Attoui, H. - Šimo, L.
Cholinergic axons regulate type I acini in salivary glands of Ixodes ricinus and Ixodes scapularis ticks.
Scientific Reports. Roč. 10, č. 1 (2020), č. článku 16054. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) EF16_019/0000759
Institucionální podpora: RVO:60077344
Klíčová slova: muscarinic acetylcholine-receptors * black-legged tick * blacklegged tick * fluid secretion * water-vapor * cattle tick * boophilus-microplus * myoinhibitory peptide * gene * expression
Obor OECD: Microbiology
Impakt faktor: 4.380, rok: 2020
Způsob publikování: Open access
https://www.nature.com/articles/s41598-020-73077-1

Regulatory factors controlling tick salivary glands (SGs) are direct upstream neural signaling pathways arising from the tick's central nervous system. Here we investigated the cholinergic signaling pathway in the SG of two hard tick species. We reconstructed the organization of the cholinergic gene locus, and then used in situ hybridization to localize mRNA encoding choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT) in specific neural cells in the Ixodes synganglion. Immunohistochemical staining revealed that cholinergic axonal projections exclusively reached type I acini in the SG of both Ixodes species. In type I acini, the rich network of cholinergic axons terminate within the basolateral infoldings of the lamellate cells. We also characterized two types (A and B) of muscarinic acetylcholine receptors (mAChRs), which were expressed in Ixodes SG. We pharmacologically assessed mAChR-A to monitor intracellular calcium mobilization upon receptor activation. In vivo injection of vesamicol-a VAChT blocker-at the cholinergic synapse, suppressed forced water uptake by desiccated ticks, while injection of atropine, an mAChR-A antagonist, did not show any effect on water volume uptake. This study has uncovered a novel neurotransmitter signaling pathway in Ixodes SG, and suggests its role in water uptake by type I acini in desiccated ticks.
Trvalý link: http://hdl.handle.net/11104/0316443