A Study on the Prevalence and Subtype Diversity of the Intestinal ProtistBlastocystissp. in a Gut-Healthy Human Population in the Czech Republic

0538647 - BC 2021 RIV CH eng J - Článek v odborném periodiku
Lhotská, Zuzana - Jirků, Milan - Hložková, Oldřiška - Brožová, Kristýna - Jirsová, Dagmar - Stensvold, C.R. - Kolísko, Martin - Jirků-Pomajbíková, Kateřina
A Study on the Prevalence and Subtype Diversity of the Intestinal ProtistBlastocystissp. in a Gut-Healthy Human Population in the Czech Republic.
Frontiers in Cellular and Infection Microbiology. Roč. 10, OCT 6 2020 (2020), č. článku 544335. ISSN 2235-2988. E-ISSN 2235-2988
Grant CEP: GA MŠMT(CZ) EF16_019/0000759
Grant ostatní: Human Frontier Science Programme(FR) RGY0078/2015
Institucionální podpora: RVO:60077344
Klíčová slova: genetic diversity * blastocystis subtypes * geographic-distribution * animals * common * epidemiology * phylogenies * microbiota * parasites * children * Blastocystis * prevalence * Czech Republic * demography * survey * genetic diversity
Obor OECD: Microbiology
Impakt faktor: 5.293, rok: 2020
Způsob publikování: Open access
https://www.frontiersin.org/articles/10.3389/fcimb.2020.544335/full

Blastocystissp. is a common intestinal protist colonizing the human intestine the prevalence of which varies across non-industrialized and industrialized countries. Its role in the human gut ecosystem remains unclear due to persisting gaps in knowledge of epidemiology and factors affecting gut colonization. Here, we aimed to expand the knowledge of the epidemiology ofBlastocystissp. in the gut-healthy humans in one of the industrialized European countries, including the distribution of its subtypes, the correlation between its occurrence and several factors such as lifestyle, contact with animals, age, and sex. A total of 288 stool samples were obtained from asymptomatic individuals over the entire age-range and 136 samples from animals with which the volunteers were in frequent contact. All samples were examined in parallel by PCR and xenicin vitroculture.Blastocystissp. was detected in samples from both human and non-human hosts. In humans, the overall prevalence was 24% and eight subtypes were found, in animals, the prevalence was 10%, and only five subtypes were detected. A higher incidence ofBlastocystissp. was observed in individuals (i) traveling outside Europe, (ii) in frequent contact with livestock, and (iii) over 50 years of age. We found no effect on gender onBlastocystissp. colonization. This study provides data on the prevalence and diversity of the gut protistBlastocystissp. and its subtypes in a gut-healthy human population with emphasis on several factors such as contact with animals, lifestyle, age, and gender.
Trvalý link: http://hdl.handle.net/11104/0316431