Počet záznamů: 1
Visualization of Lokiarchaeia and Heimdallarchaeia (Asgardarchaeota) by fluorescence in situ hybridization and catalyzed reporter deposition (CARD-FISH)
- 1.0538502 - BC 2021 RIV US eng J - Článek v odborném periodiku
Salcher, Michaela M. - Andrei, Adrian-Stefan - Bulzu, Paul-Adrian - Keresztes, Z.G. - Banciu, H.L. - Ghai, Rohit
Visualization of Lokiarchaeia and Heimdallarchaeia (Asgardarchaeota) by fluorescence in situ hybridization and catalyzed reporter deposition (CARD-FISH).
mSphere. Roč. 5, č. 4 (2020), s. 1-10, č. článku e00686-20. ISSN 2379-5042. E-ISSN 2379-5042
Grant CEP: GA ČR(CZ) GX20-12496X; GA ČR(CZ) GA17-04828S; GA ČR(CZ) GA19-23469S
Grant ostatní: AV ČR(CZ) MSM200961801
Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
Institucionální podpora: RVO:60077344
Klíčová slova: 16s ribosomal RNA * bacterioplankton populations * identification * archaea * abundant * Asgardarchaeota * Heimdallarchaeia * Lokiarchaeia * card-fish * morphology
Obor OECD: Microbiology
Impakt faktor: 4.389, rok: 2020
Způsob publikování: Open access
https://doi.org/10.1128/mSphere.00686-20
Metagenome-assembled genomes (MAGs) of Asgardarchaeota have been recovered from a variety of habitats, broadening their environmental distribu-tion and providing access to the genetic makeup of this archaeal lineage. The recent success in cultivating the first representative of Lokiarchaeia was a breakthrough in science at large and gave rise to new hypotheses about the evolution of eukaryotes. Despite their singular phylogenetic position at the base of the eukaryotic tree of life, the morphology of these bewildering organisms remains a mystery, except for the report of an unusual morphology with long, branching protrusions of the cultivated Lokiarchaeion strain 'Candidatus Prometheoarchaeum syntrophicum' MK-D1. In order to visualize this elusive group, we applied a combination of fluorescence in situ hybridization and catalyzed reporter deposition (CARD-FISH) and epifluorescence microscopy on coastal hypersaline sediment samples, using specifically designed CARD-FISH probes for Heimdallarchaeia and Lokiarchaeia lineages, and provide the first visual evidence for Heimdallarchaeia and new images of a lineage of Lokiarchaeia that is different from the cultured representative. Here, we show that while Heimdallarchaeia are characterized by a uniform cellular morphology typified by a centralized DNA localization, Lokiarchaeia display a plethora of shapes and sizes that likely reflect their broad phylogenetic diversity and ecological distribution.
Trvalý link: http://hdl.handle.net/11104/0316323
Název souboru Staženo Velikost Komentář Verze Přístup SALCHER mSphere 2020.pdf 1 1.4 MB Autorský preprint povolen
Počet záznamů: 1