Methodological considerations for monitoring soil/litter arthropods in tropical rainforests using DNA metabarcoding, with a special emphasis on ants, springtails and termites

0538463 - BC 2021 RIV BG eng J - Článek v odborném periodiku
Basset, Yves - Donoso, D. A. - Hajibabaei, M. - Wright, M. T. G. - Perez, K. H. J. - Lamarre, Greg P. A. - De León, L. F. - Palacios-Vargas, J.G. - Castaňo-Meneses, G. - Rivera, M. - Perez, F. - Bobadilla, R. - Lopez, Y. - Ramirez, J. A. - Barrios, H.
Methodological considerations for monitoring soil/litter arthropods in tropical rainforests using DNA metabarcoding, with a special emphasis on ants, springtails and termites.
Metabarcoding and Metagenomics. Roč. 4, DEC 31 (2020), s. 151-163, č. článku e58572. E-ISSN 2534-9708
Grant CEP: GA ČR(CZ) GA20-31295S; GA ČR(CZ) GJ19-15645Y
Institucionální podpora: RVO:60077344
Klíčová slova: Barro Colorado Island * Berlese-Tullgren * Collembola
Obor OECD: Entomology
Způsob publikování: Open access
https://mbmg.pensoft.net/article/58572/download/pdf/492822

Robust data to refute or support claims of global insect decline are currently lacking, particularly for the soil fauna in the tropics. DNA metabarcoding represents a powerful approach for rigorous spatial and temporal monitoring of the taxonomically challenging soil fauna. Here, we provide a detailed field protocol, which was successfully applied in Barro Colorado Island (BCI) in Panama, to collect soil samples and arthropods in a tropical rainforest, to be later processed with metabarcoding. We also estimate the proportion of soil/litter ant, springtail and termite species from the local fauna that can be detected by metabarcoding samples obtained either from Berlese-Tullgren (soil samples), Malaise or light traps. Each collecting method detected a rather distinct fauna. Soil and Malaise trap samples detected 213 species (73%) of all target species. Malaise trap samples detected many ant species, whereas soil samples were more efficient at detecting springtail and termite species. With respect to long-term monitoring of soil-dwelling and common species (more amenable to statistical trends), the best combination of two methods were soil and light trap samples, detecting 94% of the total of common species. A protocol including 100 soil, 40 Malaise and 80 light trap samples annually processed by metabarcoding would allow the long-term monitoring of at least 11%, 18% and 16% of species of soil/litter ants, springtails and termites, respectively, present on BCI, and a high proportion of the total abundance (up to 80% of all individuals) represented by these taxa.
Trvalý link: http://hdl.handle.net/11104/0317516