In vitro elucidation of suppression effects of composts to soil-borne pathogen Phytophthora nicotianaeon pepper plants using 16S amplicon sequencing and metaproteomics

0538451 - MBÚ 2021 RIV GB eng J - Článek v odborném periodiku
Ros, M. - Blaya, J. - Baldrian, Petr - Bastida, F. - Richnow, H. H. - Jehmlich, N. - Pascual, J. A.
In vitro elucidation of suppression effects of composts to soil-borne pathogen Phytophthora nicotianaeon pepper plants using 16S amplicon sequencing and metaproteomics.
Renewable Agriculture and Food Systems. Roč. 35, č. 2 (2020), s. 206-214, č. článku S1742170518000467. ISSN 1742-1705. E-ISSN 1742-1713
Institucionální podpora: RVO:61388971
Klíčová slova: Compost * metaproteomics * Phytophthora nicotianae * sequencing * suppressive
Obor OECD: Microbiology
Impakt faktor: 2.657, rok: 2020
Způsob publikování: Omezený přístup
https://www.cambridge.org/core/journals/renewable-agriculture-and-food-systems/issue/77E5E37D8A5D35F5E3253C94E2745AD8

Compost production is a critical component of organic waste management. One of the most important properties of compost is its ability to suppress soil-borne pathogens such asPhytophthora nicotianaein pepper plants. Both the physico-chemical and biological properties of composts can be responsible for the suppression of pathogens, although biological properties are the main driver. In this study, we analyzed composts with various levels of suppressiveness againstP. nicotianae. We analyzed both physico-chemical properties like pH and electrical conductivity and biological properties like microbial activity, amplicon sequencing and metaproteomics. We believed that the link between community structures and proteins could provide deep insights into the mechanism of compost suppressiveness. Our results indicate that there are differences between suppressive and non-suppressive composts at the phylogenetic level (sequencing) and at the functional level (based on analysis of the cluster of orthologous groups, COGs). The proteins identified were assigned to the carbohydrate process, cell wall structure and inorganic ion transport and metabolism.Proteobacteriacould also be new indicators ofP. nicotianaesuppression.
Trvalý link: http://hdl.handle.net/11104/0316241