Schizorhodopsins: A family of rhodopsins from Asgard archaea that function as light-driven inward H+ pumps

0538424 - BC 2021 RIV US eng J - Článek v odborném periodiku
Inoue, K. - Tsunoda, S.P. - Singh, M. - Tomida, S. - Hososhima, S. - Konno, M. - Nakamura, R. - Watanabe, H. - Bulzu, P.A. - Banciu, H.L. - Andrei, Adrian-Stefan - Uchihashi, T. - Ghai, Rohit - Béja, O. - Kandori, H.
Schizorhodopsins: A family of rhodopsins from Asgard archaea that function as light-driven inward H+ pumps.
Science Advances. Roč. 6, č. 15 (2020), s. 1-12, č. článku eaaz2441. ISSN 2375-2548. E-ISSN 2375-2548
Grant CEP: GA ČR(CZ) GA17-04828S
Grant ostatní: AV ČR(CZ) MSM200961801
Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
Institucionální podpora: RVO:60077344
Klíčová slova: resonance raman * proton pump * bacteriorhodopsin * transport * viruses
Obor OECD: Microbiology
Impakt faktor: 14.143, rok: 2020
Způsob publikování: Open access
https://doi.org/10.1126/sciadv.aaz2441

Schizorhodopsins (SzRs), a rhodopsin family first identified in Asgard archaea, the archaeal group closest to eukaryotes, are present at a phylogenetically intermediate position between typical microbial rhodopsins and heliorhodopsins. However, the biological function and molecular properties of SzRs have not been reported. Here, SzRs from Asgardarchaeota and from a yet unknown microorganism are expressed in Escherichia coil and mammalian cells, and ion transport assays and patch clamp analyses are used to demonstrate SzR as a novel type of light-driven inward H+ pump. The mutation of a cyto-plasmic glutamate inhibited inward H+ transport, suggesting that it functions as a cytoplasmic H+ acceptor. The function, trimeric structure, and H+ transport mechanism of SzR are similar to that of xenorhodopsin (XeR), a light-driven inward H+ pumping microbial rhodopsins, implying that they evolved convergently. The inward H+ pump function of SzR provides new insight into the photobiological life cycle of the Asgardarchaeota.
Trvalý link: http://hdl.handle.net/11104/0316223