Intrinsically disordered protein domain of human ameloblastin in synthetic fusion with calmodulin increases calmodulin stability and modulates its function

0538306 - ÚOCHB 2022 RIV GB eng J - Článek v odborném periodiku
Zouharová, Monika - Vymětal, Jiří - Bednárová, Lucie - Vaněk, O. - Herman, P. - Vetýšková, Veronika - Poštulková, Klára - Lingstaadas, P. S. - Vondrášek, Jiří - Boušová, Kristýna
Intrinsically disordered protein domain of human ameloblastin in synthetic fusion with calmodulin increases calmodulin stability and modulates its function.
International Journal of Biological Macromolecules. Roč. 168, Jan 31 (2021), s. 1-12. ISSN 0141-8130. E-ISSN 1879-0003
Výzkumná infrastruktura: e-INFRA CZ - 90140
Institucionální podpora: RVO:61388963
Klíčová slova: calmodulin * fusion protein * intrinsically disordered protein (IDP) * ameloblastin
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 8.025, rok: 2021
Způsob publikování: Omezený přístup
https://doi.org/10.1016/j.ijbiomac.2020.11.216

Constantly increasing attention to bioengineered proteins has led to the rapid development of new functional targets. Here we present the biophysical and functional characteristics of the newly designed CaM/AMBN-Ct fusion protein. The two-domain artificial target consists of calmodulin (CaM) and ameloblastin C-terminus (AMBN-Ct). CaM as a well-characterized calcium ions (Ca2+) binding protein offers plenty of options in terms of Ca2+ detection in biomedicine and biotechnologies. Highly negatively charged AMBN-Ct belongs to intrinsically disordered proteins (IDPs). CaM/AMBN-Ct was designed to open new ways of communication synergies between the domains with potential functional improvement. The character and function of CaM/AMBN-Ct were explored by biophysical and molecular modelling methods. Experimental studies have revealed increased stability and preserved CaM/AMBN-Ct function. The results of molecular dynamic simulations (MDs) outlined different interface patterns between the domains with potential allosteric communication within the fusion.
Trvalý link: http://hdl.handle.net/11104/0316306