Počet záznamů: 1  

A mosquito juvenile hormone binding protein (mJHBP) regulates the activation of innate immune defenses and hemocyte development

  1. 1.
    0538272 - BC 2021 RIV US eng J - Článek v odborném periodiku
    Kim, I.H. - Castillo, J.C. - Aryan, A. - Martin-Martin, I. - Nouzová, Marcela - Noriega, F. G. - Barletta, A.B.F. - Calvo, E. - Adelman, Z.N. - Ribeiro, J.M.C. - Andersen, J. F.
    A mosquito juvenile hormone binding protein (mJHBP) regulates the activation of innate immune defenses and hemocyte development.
    PLoS Pathogens. Roč. 16, č. 1 (2020), č. článku e1008288. ISSN 1553-7366. E-ISSN 1553-7374
    Institucionální podpora: RVO:60077344
    Klíčová slova: anopheles-gambiae * embryonic origin * drosophila * blood * differentiation * expression * survival * receptor * cells
    Obor OECD: Microbiology
    Impakt faktor: 6.823, rok: 2020
    Způsob publikování: Open access
    https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1008288

    Insects rely on the innate immune system for defense against pathogens, some aspects of which are under hormonal control. Here we provide direct experimental evidence showing that the juvenile hormone-binding protein (mJHBP) of Aedes aegypti is required for the regulation of innate immune responses and the development of mosquito blood cells (hemocytes). Using an mJHBP-deficient mosquito line generated by means of CRISPR-Cas9 gene editing technology we uncovered a mutant phenotype characterized by immunosuppression at the humoral and cellular levels, which profoundly affected susceptibility to bacterial infection. Bacteria-challenged mosquitoes exhibited significantly higher levels of septicemia and mortality relative to the wild type (WT) strain, delayed expression of antimicrobial peptides (AMPs), severe developmental dysregulation of embryonic and larval hemocytes (reduction in the total number of hemocytes) and increased differentiation of the granulocyte lineage. Interestingly, injection of recombinant wild type mJHBP protein into adult females three-days before infection was sufficient to restore normal immune function. Similarly, injection of mJHBP into fourth-instar larvae fully restored normal larval/pupal hemocyte populations in emerging adults. More importantly, the recovery of normal immuno-activation and hemocyte development requires the capability of mJHBP to bind JH III. These results strongly suggest that JH III functions in mosquito immunity and hemocyte development in a manner that is perhaps independent of canonical JH signaling, given the lack of developmental and reproductive abnormalities. Because of the prominent role of hemocytes as regulators of mosquito immunity, this novel discovery may have broader implications for the understanding of vector endocrinology, hemocyte development, vector competence and disease transmission.
    Trvalý link: http://hdl.handle.net/11104/0316097

     
     
Počet záznamů: 1  

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