0538265 - BC 2021 RIV GB eng J - Článek v odborném periodiku
Kaur, Binnypreet - Záhonová, Kristýna - Valach, M. - Faktorová, Drahomíra - Prokopchuk, Galina - Burger, G. - Lukeš, Julius
Gene fragmentation and RNA editing without borders: eccentric mitochondrial genomes of diplonemids.
Nucleic Acids Research. Roč. 48, č. 5 (2020), s. 2694-2708. ISSN 0305-1048. E-ISSN 1362-4962
Grant CEP: GA MŠMT(CZ) LL1601; GA MŠMT(CZ) EF16_019/0000759
Grant ostatní: Gordon and Betty Moore Foundation(US) GBMF4983.01
Program: Science
Institucionální podpora: RVO:60077344
Klíčová slova: transcriptome * algorithm * evolution * alignment * reconstruction * generation * protists * platform * extreme * tool
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 16.971, rok: 2020
Způsob publikování: Open access
https://academic.oup.com/nar/article/48/5/2694/5699674

Diplonemids are highly abundant heterotrophic marine protists. Previous studies showed that their strikingly bloated mitochondrial genome is unique because of systematic gene fragmentation and manifold RNA editing. Here we report a comparative study of mitochondrial genome architecture, gene structure and RNA editing of six recently isolated, phylogenetically diverse diplonemid species. Mitochondria) gene fragmentation and modes of RNA editing, which include cytidine-to-uridine (C-to-U) and adenosine-to-inosine (A-to-I) substitutions and 3' uridine additions (U-appendage), are conserved across diplonemids. Yet as we show here, all these features have been pushed to their extremes in the Hemistasiidae lineage. For example, Namystynia karyoxenos has its genes fragmented into more than twice as many modules than other diplonemids, with modules as short as four nucleotides. Furthermore, we detected in this group multiple A-appendage and guanosine-to-adenosine (G-to-A) substitution editing events not observed before in diplonemids and found very rarely elsewhere. With >1,000 sites, C-to-U and A-to-I editing in Namystynia is nearly 10 times more frequent than in other diplonemids. The editing density of 12% in coding regions makes Namystynia's the most extensively edited transcriptome described so far. Diplonemid mitochondrial genome architecture, gene structure and post-transcriptional processes display such high complexity that they challenge all other currently known systems.
Trvalý link: http://hdl.handle.net/11104/0316093