Wnt/beta-catenin signaling is an evolutionarily conserved determinant of chordate dorsal organizer

0538170 - ÚMG 2021 RIV GB eng J - Článek v odborném periodiku
Kozmiková, Iryna - Kozmik, Zbyněk
Wnt/beta-catenin signaling is an evolutionarily conserved determinant of chordate dorsal organizer.
eLife. Roč. 9, May (2020), č. článku e56817. ISSN 2050-084X. E-ISSN 2050-084X
Grant CEP: GA ČR GC15-21285J; GA ČR GA17-15374S; GA MŠMT(CZ) EF16_013/0001775
Institucionální podpora: RVO:68378050
Klíčová slova: nuclear beta-catenin * branchiostoma-belcheri-tsingtauense * right asymmetric development * glycogen-synthase kinase-3 * nodal-related signals * xenopus embryos * axis formation * mesoderm induction * ventral axis * cell fates
Obor OECD: Developmental biology
Impakt faktor: 8.146, rok: 2020
Způsob publikování: Open access
https://elifesciences.org/articles/56817

Deciphering the mechanisms of axis formation in amphioxus is a key step to understanding the evolution of chordate body plan. The current view is that Nodal signaling is the only factor promoting the dorsal axis specification in the amphioxus, whereas Wnt/beta-catenin signaling plays no role in this process. Here, we re-examined the role of Wnt/beta catenin signaling in the dorsal/ventral patterning of amphioxus embryo. We demonstrated that the spatial activity of Wnt/beta-catenin signaling is located in presumptive dorsal cells from cleavage to gastrula stage, and provided functional evidence that Wnt/beta-catenin signaling is necessary for the specification of dorsal cell fate in a stage-dependent manner. Microinjection of Wnt8 and Wnt11 mRNA induced ectopic dorsal axis in neurulae and larvae. Finally, we demonstrated that Nodal and Wnt/beta-catenin signaling cooperate to promote the dorsal-specific gene expression in amphioxus gastrula. Our study reveals high evolutionary conservation of dorsal organizer formation in the chordate lineage.
Trvalý link: http://hdl.handle.net/11104/0315991