A chicken embryo model for the maintenance and amplification of Cryptosporidium parvum and Cryptosporidium baileyi oocysts

0537506 - BC 2021 RIV DE eng J - Článek v odborném periodiku
Holubová, Nikola - Sak, Bohumil - Schulzova, T. - Konečný, R. - Rost, M. - Tůmová, Lenka - McEvoy, J. - Kváč, Martin
A chicken embryo model for the maintenance and amplification of Cryptosporidium parvum and Cryptosporidium baileyi oocysts.
European Journal of Protistology. Roč. 75, AUG 2020 (2020), č. článku 125718. ISSN 0932-4739. E-ISSN 1618-0429
Grant CEP: GA MŠMT(CZ) LM2015062; GA ČR(CZ) GA18-12364S
Institucionální podpora: RVO:60077344
Klíčová slova: n. apicomplexa cryptosporidiidae * intestinal cryptosporidiosis * infection * giardia * birds * age * cysts * host * spp. * parasites * Inoculation * In ovo * Chicken embryo * Egg * Model host * Shedding
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 3.020, rok: 2020
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/pii/S0932473920300481?via%3Dihub

Cryptosporidium is a genus of apicomplexan parasites that inhabit the respiratory and gastrointestinal tracts of vertebrates. Research of these parasites is limited by a lack of model hosts. This study aimed to determine the extent to which infection at the embryo stage can enhance the propagation of Cryptosporidium oocysts in chickens. Nine-day-old chicken embryos and one day-old chickens were experimentally infected with different doses of Cryptosporidium baileyi and Cryptosporidium parvum oocysts. Post hatching, all chickens had demonstrable infections, and the infection dose had no effect on the course of infection. Chickens infected as embryos shed oocysts immediately after hatching and shed significantly more oocysts over the course of the infection than chickens infected as one-day-olds. In chickens infected as embryos, C. baileyi was found in all organs except the brain whereas, C. parvum was only found in the gastrointestinal tract and trachea. In chickens infected as one-day-olds, C. baileyi was only found in the gastrointestinal tract and trachea. Chickens infected as embryos with C. baileyi died within 16 days of hatching. All other chickens cleared the infection. Infection of chickens as embryos could be used as an effective and simple model for the propagation of C. baileyi and C. parvum. (C) 2020 Elsevier GmbH. All rights reserved.
Trvalý link: http://hdl.handle.net/11104/0315325