Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies

0537498 - BC 2021 RIV CH eng J - Článek v odborném periodiku
Helmova, R. - Hönig, Václav - Tykalová, Hana - Palus, Martin - Bell-Sakyi, L. - Grubhoffer, Libor
Tick-Borne Encephalitis Virus Adaptation in Different Host Environments and Existence of Quasispecies.
Viruses. Roč. 12, č. 8 (2020), č. článku 902. E-ISSN 1999-4915
Grant CEP: GA MŠMT(CZ) LTARF18021; GA ČR(CZ) GA20-30500S; GA ČR(CZ) GA18-27204S
Institucionální podpora: RVO:60077344
Klíčová slova: nonstructural protein ns2a * amino-acid substitution * west-nile-virus * swiss-model * subcellular-localization * 3'-untranslated region * envelope glycoprotein * noncoding region * cell-lines * dengue * tbev * host alternation * neuroinvasiveness * genome mutation * quasispecies * flavivirus adaptation * tick cell line
Obor OECD: Microbiology
Impakt faktor: 5.048, rok: 2020
Způsob publikování: Open access
https://www.mdpi.com/1999-4915/12/8/902

A highly virulent strain (Hypr) of tick-borne encephalitis virus (TBEV) was serially subcultured in the mammalian porcine kidney stable (PS) andIxodes ricinustick (IRE/CTVM19) cell lines, producing three viral variants. These variants exhibited distinct plaque sizes and virulence in a mouse model. Comparing the full-genome sequences of all variants, several nucleotide changes were identified in different genomic regions. Furthermore, different sequential variants were revealed to co-exist within one sample as quasispecies. Interestingly, the above-mentioned nucleotide changes found within the whole genome sequences of the new variants were present alongside the nucleotide sequence of the parental strain, which was represented as a minority quasispecies. These observations further imply that TBEV exists as a heterogeneous population that contains virus variants pre-adapted to reproduction in different environments, probably enabling virus survival in ticks and mammals.
Trvalý link: http://hdl.handle.net/11104/0315319