Ionic Environment Affects Biomolecular Interactions of Amyloid-beta: SPR Biosensor Study

0537414 - ÚFE 2021 RIV CH eng J - Článek v odborném periodiku
Hemmerová, Erika - Špringer, Tomáš - Krištofíková, Z. - Homola, Jiří
Ionic Environment Affects Biomolecular Interactions of Amyloid-beta: SPR Biosensor Study.
International Journal of Molecular Sciences. Roč. 21, č. 24 (2020), č. článku 9727. E-ISSN 1422-0067
Grant CEP: GA ČR(CZ) GA19-02739S; GA ČR(CZ) GX20-23787X
Institucionální podpora: RVO:67985882
Klíčová slova: 17beta-hydroxysteroid dehydrogenase 10 * Amyloid beta * Biomolecular interactions * Mitochondrial matrix
Obor OECD: Optics (including laser optics and quantum optics)
Impakt faktor: 5.924, rok: 2020
Způsob publikování: Open access
https://doi.org/10.3390/ijms21249727

In early stages of Alzheimer's disease (AD), amyloid beta (A beta) accumulates in the mitochondrial matrix and interacts with mitochondrial proteins, such as cyclophilin D (cypD) and 17 beta-hydroxysteroid dehydrogenase 10 (17 beta-HSD10). Multiple processes associated with AD such as increased production or oligomerization of A beta affect these interactions and disbalance the equilibrium between the biomolecules, which contributes to mitochondrial dysfunction. Here, we investigate the effect of the ionic environment on the interactions of A beta (A beta(1-40), A beta(1-42)) with cypD and 17 beta-HSD10 using a surface plasmon resonance (SPR) biosensor. We show that changes in concentrations of K+ and Mg2+ significantly affect the interactions and may increase the binding efficiency between the biomolecules by up to 35% and 65% for the interactions with A beta(1-40) and A beta(1-42), respectively, in comparison with the physiological state. We also demonstrate that while the binding of A beta(1-40) to cypD and 17 beta-HSD10 takes place preferentially around the physiological concentrations of ions, decreased concentrations of K+ and increased concentrations of Mg2+ promote the interaction of both mitochondrial proteins with A beta(1-42). These results suggest that the ionic environment represents an important factor that should be considered in the investigation of biomolecular interactions taking place in the mitochondrial matrix under physiological as well as AD-associated conditions
Trvalý link: http://hdl.handle.net/11104/0315128