Počet záznamů: 1  

Genetic tool development in marine protists: emerging model organisms for experimental cell biology

  1. 1.
    0537241 - BC 2021 RIV GB eng J - Článek v odborném periodiku
    Faktorová, Drahomíra - Nisbet, R.E.R. - Robledo, J.A.F. - Casacuberta, E. - Sudek, L. - Allen, A. E. - Ares, M. - Areste, C. - Balestreri, C. - Barbrook, A.C. - Beardslee, P. - Bender, S. - Booth, D.S. - Bowler, C. - Breglia, S.A. - Brownlee, C. - Burger, G. - Cerutti, H. - Cesaroni, R. - Chiurillo, M.A. - Clemente, T. - Coles, D.B. - Collier, J.L. - Cooney, E.C. - Coyne, K. - Docampo, R. - Dupont, C.L. - Edgcomb, V. - Einarsson, E. - Elustondo, P.A. - Federici, F. - Freire-Beneitez, V. - Freyria, N.J. - Fukuda, K. - Garcia, P.A. - Girguis, P.R. - Gomaa, F. - Hampl, V. - Kachale, Ambar - Kaur, Binnypreet - Keeling, P. J. - Vanclová-Novák, A.M.G. - Pyrih, J. - Lukeš, Julius … celkem 113 autorů
    Genetic tool development in marine protists: emerging model organisms for experimental cell biology.
    Nature Methods. Roč. 17, č. 5 (2020), s. 481-494. ISSN 1548-7091. E-ISSN 1548-7105
    Grant CEP: GA MŠMT(CZ) EF16_019/0000759
    Grant ostatní: Gordon and Betty Moore Foundation(US) GMBM4982.01
    Institucionální podpora: RVO:60077344
    Klíčová slova: homologous recombination * transformation * genome
    Obor OECD: Microbiology
    Impakt faktor: 28.547, rok: 2020
    Způsob publikování: Open access
    https://www.nature.com/articles/s41592-020-0796-x

    Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.
    Trvalý link: http://hdl.handle.net/11104/0314982

     
     
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.