Počet záznamů: 1  

LSD1 prevents aberrant heterochromatin formation in Neurospora crassa

  1. 1.
    0537077 - ÚOCHB 2021 RIV GB eng J - Článek v odborném periodiku
    Storck, W. K. - Bicocca, V. T. - Rountree, M. R. - Honda, S. - Ormsby, Tereza - Selker, E. U.
    LSD1 prevents aberrant heterochromatin formation in Neurospora crassa.
    Nucleic Acids Research. Roč. 48, č. 18 (2020), s. 10199-10210. ISSN 0305-1048. E-ISSN 1362-4962
    Institucionální podpora: RVO:61388963
    Klíčová slova: DNA methylation * histone H3 * facultative heterochromatin
    Obor OECD: Biochemistry and molecular biology
    Impakt faktor: 16.971, rok: 2020
    Způsob publikování: Open access
    https://doi.org/10.1093/nar/gkaa724

    Heterochromatin is a specialized form of chromatin that restricts access to DNA and inhibits genetic processes, including transcription and recombination. In Neurospora crassa, constitutive heterochromatin is characterized by trimethylation of lysine 9 on histone H3, hypoacetylation of histones, and DNA methylation. We explored whether the conserved histone demethylase, lysine-specific demethylase 1 (LSD1), regulates heterochromatin in Neurospora, and if so, how. Though LSD1 is implicated in heterochromatin regulation, its function is inconsistent across different systems, orthologs of LSD1 have been shown to either promote or antagonize heterochromatin expansion by removing H3K4me or H3K9me respectively. We identify three members of the Neurospora LSD complex (LSDC): LSD1, PHF1, and BDP-1. Strains deficient for any of these proteins exhibit variable spreading of heterochromatin and establishment of new heterochromatin domains throughout the genome. Although establishment of H3K9me3 is typically independent of DNA methylation in Neurospora, instances of DNA methylation-dependent H3K9me3 have been found outside regions of canonical heterochromatin. Consistent with this, the hyper-H3K9me3 phenotype of Δlsd1 strains is dependent on the presence of DNA methylation, as well as HCHC-mediated histone deacetylation, suggesting that spreading is dependent on some feedback mechanism. Altogether, our results suggest LSD1 works in opposition to HCHC to maintain proper heterochromatin boundaries.
    Trvalý link: http://hdl.handle.net/11104/0316284

     
     
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.