Additive Effects of Omega-3 Fatty Acids and Thiazolidinediones in Mice Fed a High-Fat Diet: Triacylglycerol/Fatty Acid Cycling in Adipose Tissue

0536873 - FGÚ 2021 RIV CH eng J - Článek v odborném periodiku
Bardová, Kristina - Funda, Jiří - Pohl, Radek - Čajka, Tomáš - Hensler, Michal - Kuda, Ondřej - Janovská, Petra - Adamcová, Kateřina - Irodenko, Ilaria - Leňková, Lucie - Zouhar, Petr - Horáková, Olga - Flachs, Pavel - Rossmeisl, Martin - Colca, J. - Kopecký, Jan
Additive Effects of Omega-3 Fatty Acids and Thiazolidinediones in Mice Fed a High-Fat Diet: Triacylglycerol/Fatty Acid Cycling in Adipose Tissue.
Nutrients. Roč. 12, č. 12 (2020), č. článku 3737. E-ISSN 2072-6643
Grant CEP: GA ČR(CZ) GA19-02411S
Institucionální podpora: RVO:67985823 ; RVO:61388963
Klíčová slova: insulin * lipogenesis * obesity * glucose homeostasis * adipocytes
Obor OECD: Physiology (including cytology); Analytical chemistry (UOCHB-X)
Impakt faktor: 5.719, rok: 2020
Způsob publikování: Open access
https://www.mdpi.com/2072-6643/12/12/3737

Long-chain n-3 polyunsaturated fatty acids (Omega-3) and anti-diabetic drugs thiazolidinediones (TZDs) exhibit additive effects in counteraction of dietary obesity and associated metabolic dysfunctions in mice. The underlying mechanisms need to be clarified. Here, we aimed to learn whether the futile cycle based on the hydrolysis of triacylglycerol and re-esterification of fatty acids (TAG/FA cycling) in white adipose tissue (WAT) could be involved. We compared Omega-3 (30 mg/g diet) and two different TZDs—pioglitazone (50 mg/g diet) and a second-generation TZD, MSDC-0602K (330 mg/g diet)—regarding their effects in C57BL/6N mice fed an obesogenic high-fat (HF) diet for 8 weeks. The diet was supplemented or not by the tested compound alone or with the two TZDs combined individually with Omega-3. Activity of TAG/FA cycle in WAT was suppressed by the obesogenic HF diet. Additive effects in partial rescue of TAG/FA cycling in WAT were observed with both combined interventions, with a stronger effect of Omega-3 and MSDC-0602K. Our results (i) supported the role of TAG/FA cycling in WAT in the beneficial additive effects of Omega-3 and TZDs on metabolism of diet-induced obese mice, and (ii) showed differential modulation of WAT gene expression and metabolism by the two TZDs, depending also on Omega-3.
Trvalý link: http://hdl.handle.net/11104/0314630